ZFIN ID: ZDB-FIG-060707-3
Goishi et al., 2006 - {alpha}A-crystallin expression prevents {gamma}-crystallin insolubility and cataract formation in the zebrafish cloche mutant lens. Development (Cambridge, England)   133(13):2585-2593 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Long-pec to Day 4

Fig. 2 crystallin gene expression. (A) Zebrafish embryo (2.5 dpf) mRNA expression levels were analyzed by semi-quantitative RT-PCR. Lane 1, wild type; lane 2, cloche. Wild-type versus cloche gene expression is shown for αA-crystallin, αBb-crystallin, ßB1-crystallin, γ-crystallin and flk1 (vegfr2). ef1a was used as a loading control. (B) &alphaA-crystallin promoter-EGFP was injected into one- to four-cell stage embryos and fluorescence (green) was measured at 2.5 dpf. Lateral views are shown at low and high magnifications. Fluorescence was found only in the lens and was greatly diminished in the cloche lens. (C) Western blot. Total protein as described in Fig. 1A were extracted and lysates (2 µg) were separated by SDS-PAGE and analyzed by western blotting with anti-αA-crystallin. The bands were analyzed by an image analysis program (ImageJ) and the values were normalized to wild-type bands being 100%. (D) Crystallin expression as function of development. Zebrafish embryo (2-4 dpf) mRNA expression levels were analyzed by semi-quantitative RT-PCR. Wild-type versus cloche gene expression is shown for αA-crystallin and γ-crystallin expression. ef1a was used as a loading control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cryaa WT standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism WB
Pec-fin whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism RTPCR
Pec-fin whole organism WB
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
cryabb WT standard conditions Pec-fin whole organism RTPCR
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Pec-fin whole organism RTPCR
crybb1 WT standard conditions Pec-fin whole organism RTPCR
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Pec-fin whole organism RTPCR
crygm2d1 WT standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
eef1a1l1 WT standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
kdrl WT standard conditions Pec-fin whole organism RTPCR
npas4lDf(Chr13:lycat)m39(INDO) standard conditions Pec-fin whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development