FIGURE

Fig. S2

ID

ZDB-FIG-060919-4

Publication

Goishi et al., 2006 - {alpha}A-crystallin expression prevents {gamma}-crystallin insolubility and cataract formation in the zebrafish cloche mutant lens

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

crystallin insolubility and rescue in cloche^s5. (A) Western blot: total cloche^m39 and cloche^s5 embryo extracts were analyzed by western blotting with anti-αA-crystallin antibody. (B) Zebrafish embryo (2-4 dpf) mRNA levels were analyzed by semi-quantitative RT-PCR. Wild-type versus cloche^s5 embryo gene expression is shown for αA-crystallin and γ-crystallin. ef1a was used as a loading control. (C) αA-crystallin overexpression prevents γ-crystallin insolubility. Zebrafish embryos (2.5 dpf) were lysed detergent-free in 20 mM Tris-HCl, pH 7.5, and centrifuged. Soluble (top) and insoluble (bottom) fractions were separated, and were each analyzed by western blot with anti-γ-crystallin antibody. Two independent experiments using cloche^s5 were performed.

Expression Data

Genes:	cryaa crygm2d1 eef1a1l1
Fish:	WT npas4l^s5 Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39 (INDO)
Anatomical Term:	whole organism
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development