ZFIN ID: ZDB-FIG-060919-4
Goishi et al., 2006 - {alpha}A-crystallin expression prevents {gamma}-crystallin insolubility and cataract formation in the zebrafish cloche mutant lens. Development (Cambridge, England)   133(13):2585-2593 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Long-pec to Day 4

Fig. S2 crystallin insolubility and rescue in cloches5. (A) Western blot: total clochem39 and cloches5 embryo extracts were analyzed by western blotting with anti-αA-crystallin antibody. (B) Zebrafish embryo (2-4 dpf) mRNA levels were analyzed by semi-quantitative RT-PCR. Wild-type versus cloches5 embryo gene expression is shown for αA-crystallin and γ-crystallin. ef1a was used as a loading control. (C) αA-crystallin overexpression prevents γ-crystallin insolubility. Zebrafish embryos (2.5 dpf) were lysed detergent-free in 20 mM Tris-HCl, pH 7.5, and centrifuged. Soluble (top) and insoluble (bottom) fractions were separated, and were each analyzed by western blot with anti-γ-crystallin antibody. Two independent experiments using cloches5 were performed.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cryaa WT standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism WB
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
npas4lDf(Chr13:lycat)m39 (INDO) standard conditions Pec-fin whole organism WB
npas4ls5 standard conditions Long-pec whole organism RTPCR
Pec-fin whole organism WB
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
crygm2d1 WT standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
npas4ls5 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
eef1a1l1 WT standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
npas4ls5 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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