FIGURE

Fig. 7

ID

ZDB-FIG-060522-52

Publication

Foucher et al., 2006 - Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants

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Fig. 7

Early r1 rescue in fgf8^-/-; OtxH embryos is independent of gbx2. Dorsal views, anterior towards the left showing efnb2a expression in wild-type (A, n=5/5), OtxH (B, n=8/9), wild type+SU5402 (C, n=12/15) or OtxH+SU5402 (D, n=18/23) embryos. The anteroposterior extent of r1 territory (arrows) is the same in OtxH with or without SU5402. Conversely, SU5402 treatment affects r5-7 development as previously reported (Maves et al., 2002). (E,F) Lateral views, anterior towards the left, of wild-type (E) and fgf8^-/- (F) 8-somite stage embryos, showing double expression of otx2 (blue) and her5pac:egfp (red). (G-J) Lateral views, anterior towards the left, showing gbx2 expression in wild type (G), OtxH (H), fgf8^-/- (I) or fgf8^-/-; OtxH (J). gbx2 CNS expression is lost in fgf8^-/- (arrows) and is not rescued in fgf8^-/-; OtxH (out of 21 fgf8^-/-; OtxH embryos, only five show a very faint rescue of Gbx2 expression in the very ventral metencephalic area). Asterisks indicate gbx2 expression in the epibranchial placode precursors.

Expression Data

Genes:	efnb2a gbx2 otx2b
Fish:	WT fgf8a^{ti282a/ti282a} WT + MO1-otx2a + MO1-otx2b fgf8a^{ti282a/ti282a} + MO1-otx2a + MO1-otx2b
Condition:	chemical treatment: SU5402
Knockdown Reagents:	MO1-otx2a MO1-otx2b
Anatomical Terms:	hindbrain neural keel midbrain neural rod rhombomere 1
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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