FIGURE

Fig. S3

ID

ZDB-FIG-060522-55

Publication

Foucher et al., 2006 - Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants

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Fig. S3

Expression profile of iro2a and iro1b. All prim-5 brains are lateral view, anterior to the left. Expression of iro2a (A-D) and iro1b (E-H) in wild type (A,E), OtxH (B,F), fgf8^–/– (C,G) and fgf8^–/–; OtxH (D,H) embryos. Both genes are expressed in diencephalon subdomains, midbrain (mb), r1 and hindbrain in wild type. Diencephalic and midbrain domains are, respectively, reduced or lacking in OtxH embryos. A fainter midbrain domain is expanded in fgf8^–/– embryos. Prospective cerebellar territories are iros-positive in wild type, OtxH and fgf8^–/–; OtxH embryos (arrows), while OtxH embryos might have a general slight decrease in mid/hinbrain staining.

Expression Data

Genes:	irx1b irx2a
Fish:	WT fgf8a^{ti282a/ti282a} WT + MO1-otx2a + MO1-otx2b fgf8a^{ti282a/ti282a} + MO1-otx2a + MO1-otx2b
Knockdown Reagents:	MO1-otx2a MO1-otx2b
Anatomical Terms:	diencephalon hindbrain midbrain rhombomere 1
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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