ZFIN ID: ZDB-FIG-060113-5
Rohr et al., 2006 - Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesis. Development (Cambridge, England)   133(1):107-115 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 20-25 somites

Fig. 5 Apicobasal polarity is disrupted in has/prkci and nok/mpp5 morphants. Transverse sections of heart cone stage (20-somites) embryos. The section plane is indicated in each case (dotted lines). PRKC/aPKC and <α-catenin, red or gray; ZO-1, green; GFP false colored in blue. (A) Drawing of a transverse section through the center of the heart cone. Two bilateral wings of myocardial cells are indicated in yellow. (B,B'') In wild type, ZO-1 is localized to the apical membranes of the monolayered myocardial cell layer. (B''') PRKC is localized basal of ZO-1 (see arrowheads). (C,C'') nok morphants display diffusely positioned spots of ZO-1. (C''') PRKC is mislocalized in a diffuse pattern. (D,D'',D''') Co-localization of ZO-1 and α-catenin at the apical membrane in wild type. (E,E'',E''') In has morphants, co-localization of ZO-1 and ß-catenin is lost. The peridermal localization of ZO-1 and α-catenin is not affected (arrowheads). (E''') α-catenin is diffusely distributed along the membrane. Stars indicate myocardial cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctnna1 twu34Tg standard conditions 20-25 somites heart rudiment IHC
twu34Tg + MO1-prkci standard conditions 20-25 somites heart rudiment IHC
tjp1a twu34Tg standard conditions 20-25 somites heart rudiment IHC
twu34Tg + MO1-mpp5a standard conditions 20-25 somites heart rudiment IHC
twu34Tg + MO1-prkci standard conditions 20-25 somites heart rudiment IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development