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ZIRC
ZFIN ID: ZDB-FIG-060113-1
Rohr et al., 2006 - Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesis. Development (Cambridge, England)   133(1):107-115 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-15

Fig. 1 Cardiac morphogenesis in different genetic backgrounds. (A) Depiction of several key stages of zebrafish heart formation, beginning with the juxtaposition of two bilateral cardiac progenitor cell populations along the midline of the embryo (dotted line) to the elongation of the heart tube along the anterior-posterior axis. Dorsal view onto heart field. See text for detailed explanations. (B-I) cmlc2, (J,K) vmhc and (L,M) amhc expression. Embryos are 30-32 hpf. Parts L and M were captured with a 30° angle from dorsal for better visualization of atrial morphology. (B-D) Comparison of wild-type, has/prkci morphant and Tg(cmlc2:prkci) transgenic and has/prkci morphant embryos. (E) Heart cone formation is not completed, resulting in bilateral wings of myocardial progenitor cells (arrowheads) in prkci2A kinase-dead mutants. (F,G) Heart cone `tilting' occurs, but heart tube elongation is delayed to varying degrees in nok mutants. (F) Severe and (G) medium-strength phenotypes. (H) nok/mpp5 morphants display a severe heart tube elongation defect. (I) Co-injection of nok/mpp5 MO with wild-type nok/mpp5 mRNA rescues myocardial morphogenesis. There is no developmental delay. (J,K) Length of the ventricle is affected in nok mutants. (L,M) Length of the atrium is shortened and, along the mediolateral axis, narrower than wild type. (N,O) noks305 mutants and embryos that express high levels of PRKCi2A mutant protein (overexpression phenotype) are phenotypically similar. The body axis is curved ventrally and the retinal pigmented epithelium is largely disrupted, leaving the central portion of the retina uncovered, a phenotype more severe than in has mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh6 WT standard conditions Prim-15 heart tube ISH
mpp5am520/m520(AB) standard conditions Prim-15 heart tube ISH
myh7 WT standard conditions Prim-15 heart tube ISH
mpp5am520/m520(AB) standard conditions Prim-15 heart tube ISH
myl7 WT standard conditions Prim-15 heart tube ISH
WT + MO1-mpp5a standard conditions Prim-15 heart tube ISH
WT + MO1-prkci standard conditions Prim-15 heart tube ISH
md2Tg + MO1-prkci standard conditions Prim-15 heart tube ISH
mpp5am520/m520(AB) standard conditions Prim-15 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development