ZFIN ID: ZDB-FIG-060113-4
Rohr et al., 2006 - Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesis. Development (Cambridge, England)   133(1):107-115 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to 26+ somites

Fig. 4 Myocardial epithelial organization is disrupted in has/prkci and nok/mpp5 morphants. All images represent reconstructions of confocal Z-stack sections imaged on whole mounts. (A-E,G) cmlc2:GFP, green (nuclear GFP within myocardial cells); PRKC/aPKC, red; ZO-1, blue. PRKC and ZO-1 were used as a counterstain to visualize the embryonic midline (see also dotted line in D). (F,H) cmlc2:GFP, green and ZO-1, blue. (Q-S) cmlc2:GFP and filamentous actin. (A) At the 16-somite stage, wild-type myocardial cells are organized into two bilateral sheets of cells. (B) Both sheets converge onto the midline, where they fuse to form the heart cone around the 20-somite stage. (C) Further convergence leads to a narrowing of the heart cone and expansion along the dorsoventral axis around the 24-somite stage. The entire heart cone moves out of the midline toward the left and anterior. (D) Heart cone tilting places the heart into the anterior-posterior orientation by the 28-somite stage. The atrium (arrowhead) is located to the left and anterior whereas the ventricle (arrow) is oriented toward the midline and posterior. (E,G) nok/mpp5 morphants display a delay in myocardial fusion to form the heart cone. (F,H) has/prkci morphants undergo heart cone fusion at the midline but tilting of the heart cone into the anterior-posterior orientation fails. Note the disrupted appearance of the myocardial layer, with holes and rough edges (arrows). (I-P) All images are details of confocal reconstructions of confocal Z-stacks imaged from 16-somite stage whole mounts, separated into the individual channels. (I-K) At the 16-somite stage, wild-type myocardial cells have an epithelial organization with junctional distribution of PRKC and ZO-1. (L-N) At the 16-somite stage, nok/mpp5 morphants display a diffuse distribution of PRKC along membranes and ZO-1 junctional belts are disrupted and appear as spots. (O,P) At the 16-somite stage, has/prkci morphants display a spotted distribution of ZO-1. (Q,R) Dorsal view onto the heart cone shown in S. (S) During tilting, specialized elongated myocardial cells containing actin-cables attach the heart cone to surrounding tissues (see arrow). Orientation: dorsal view and anterior to the top, except in S, lateral view and anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tjp1a twu34Tg standard conditions 14-19 somites heart primordium IHC
20-25 somites heart primordium IHC
26+ somites heart rudiment IHC
twu34Tg + MO1-mpp5a standard conditions 14-19 somites heart primordium IHC
20-25 somites heart rudiment IHC
26+ somites heart rudiment IHC
twu34Tg + MO1-prkci standard conditions 14-19 somites heart primordium IHC
20-25 somites heart rudiment IHC
26+ somites heart rudiment IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development