PUBLICATION

Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesis

Authors
Rohr, S., Bit-Avragim, N., and Abdelilah-Seyfried, S.
ID
ZDB-PUB-051207-15
Date
2006
Source
Development (Cambridge, England)   133(1): 107-115 (Journal)
Registered Authors
Abdelilah-Seyfried, Salim
Keywords
Organ morphogenesis, prkci, Myocardium, heart and soul, nagie oko, mpp5, pals1, Zebrafish
MeSH Terms
  • Animals
  • Cell Polarity/physiology*
  • Coronary Angiography
  • Guanylate Cyclase/genetics
  • Guanylate Cyclase/metabolism*
  • Heart/embryology*
  • In Situ Hybridization
  • In Situ Nick-End Labeling
  • Isoenzymes/metabolism*
  • Morphogenesis/physiology*
  • Mutagenesis, Site-Directed
  • Myocardium/cytology*
  • Myocardium/metabolism
  • Protein Kinase C/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
16319113 Full text @ Development
Abstract
Organ morphogenesis requires cellular shape changes and tissue rearrangements that occur in a precisely timed manner. Here, we show that zebrafish heart and soul (Has)/protein kinase C iota (PRKCi) is required tissue-autonomously within the myocardium for normal heart morphogenesis and that this function depends on its catalytic activity. In addition, we demonstrate that nagie oko (Nok) is the functional homolog of mammalian protein associated with Lin-seven 1 (Pals1)/MAGUK p55 subfamily member 5 (Mpp5), and we dissect its earlier and later functions during myocardial morphogenesis. Has/PRKCi and Nok/Mpp5 are required early for the polarized epithelial organization and coherence of myocardial cells during heart cone formation. Zygotic nok/mpp5 mutants have later myocardial defects, including an incomplete heart tube elongation corresponding with a failure of myocardial cells to correctly expand in size. Furthermore, we show that nok/mpp5 acts within myocardial cells during heart tube elongation. Together, these results demonstrate that cardiac morphogenesis depends on the polarized organization and coherence of the myocardium, and that the expansion of myocardial cell size contributes to the transformation of the heart cone into an elongated tube.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes