ZFIN ID: ZDB-FIG-050214-5
Dooley et al., 2005 - Zebrafish scl functions independently in hematopoietic and endothelial development. Developmental Biology   277(2):522-536 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 10-13 somites

Fig. 6 Analysis of early gene expression in the scl morphants. Expression of gata2 (A, B), lmo2 (C, D), dra (E, F) and fli1b (I, J) at the 10- to 12-somite stage in scl morphants and uninjected controls. As an internal control, the gata2 ISH was performed as a double with myoD, a marker of the somitic tissue, detected in red (A, B). (I, J) Double ISH using probes for lmo2 (red arrow) and fli1b (green arrow) in uninjected and scl morphant embryo, showing higher magnification of posterior mesoderm. All embryos were flat mounted and shown with anterior to the left. In D, a schematic illustrates the orientation of embryos and regions of erythroid progenitors and angioblasts.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
drl WT standard conditions 10-13 somites lateral mesoderm ISH
WT + MO1-tal1 + MO2-tal1 standard conditions 10-13 somites lateral mesoderm ISH
gata2a WT standard conditions 10-13 somites lateral mesoderm ISH
WT + MO1-tal1 + MO2-tal1 standard conditions 10-13 somites lateral mesoderm ISH
lmo2 WT standard conditions 10-13 somites lateral mesoderm ISH
WT + MO1-tal1 + MO2-tal1 standard conditions 10-13 somites lateral mesoderm ISH
myod1 WT standard conditions 10-13 somites somite ISH
WT + MO1-tal1 + MO2-tal1 standard conditions 10-13 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 277(2), Dooley, K.A., Davidson, A.J., and Zon, L.I., Zebrafish scl functions independently in hematopoietic and endothelial development, 522-536, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.