ZFIN ID: ZDB-FIG-050208-2
Dooley et al., 2005 - Zebrafish scl functions independently in hematopoietic and endothelial development. Developmental Biology   277(2):522-536 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 2 Loss of embryonic erythroid development inscl morphants. (A, B) Loss of gata1 expression as detected by ISH in the scl morphants at the 10-somite stage. (C) Co-injection of 300 pg of scl RNA rescued the gata1 expression. Loss of transcripts for runx1 (D, E) and c-myb (F, G) at the 10 somite stage, and βe3-globin transcripts at 24 hpf (H, I), in scl morphants. (J, K) Absence of circulating erythroid cells in the scl morphant embryos (arrow), in comparison to uninjected control embryos, as visualized under a dissecting microscope. Whole (A–C, H–I) or flat-mounted (D–G) embryos are shown with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a WT standard conditions 10-13 somites lateral mesoderm ISH
WT + MO1-tal1 + MO2-tal1 standard conditions 10-13 somites Not Detected lateral mesoderm ISH
hbbe3 WT standard conditions Prim-5 unspecified ISH
WT + MO1-tal1 + MO2-tal1 standard conditions Prim-5 unspecified ISH
myb WT standard conditions 10-13 somites lateral mesoderm ISH
WT + MO1-tal1 + MO2-tal1 standard conditions 10-13 somites Not Detected lateral mesoderm ISH
runx1 WT standard conditions 10-13 somites lateral mesoderm ISH
WT + MO1-tal1 + MO2-tal1 standard conditions 10-13 somites Not Detected lateral mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 277(2), Dooley, K.A., Davidson, A.J., and Zon, L.I., Zebrafish scl functions independently in hematopoietic and endothelial development, 522-536, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.