Fig. 1

The expression of igf1 during early development of zebrafish. (A) Real-time PCR results showing the temporal expression of igf1 relative to a housekeeping gene (18s) in zebrafish embryos during the first 72 hpf; (B–O) Results of whole mount in situ hybridization showing the spatial expression of igf1 during embryogenesis. (B and C) 4 hpf embryo (sphere stage), lateral and animal view; (D and E) 6 hpf (shield stage), lateral and animal view; (F and G) 18 hpf (18-somite stage), lateral and animal view; (H and I) 24 hpf (prim-5 stage), lateral and dorsal view; (J, K and L) 48 hpf (long-pec stage), lateral and dorsal view; (M, N and O) 72 hpf (protruding-mouth stage), lateral and ventral view. TG, trigeminal ganglia; OV, otic vesicle; Ls, lens.
Fig. 2

The expression of igf2a during early development of zebrafish. (A) Real-time PCR results showing the temporal expression of igf2a relative to a housekeeping gene (18s) in zebrafish embryos during the first 72 hpf; (B–L) Results of whole mount in situ hybridization showing the spatial expression of igf2a during embryogenesis. (B and C) 10 hpf (bud stage), lateral and animal view; (D and E) 14 hpf (10-somite stage), lateral and animal view; (F and G) 16 hpf (14-somite stage), lateral and animal view; (H and I) 18 hpf (18-somite stage), lateral and animal view; (J) 24 hpf (prim-5 stage), lateral view; (K) 48 hpf (long-pec stage), lateral view; (L) 72 hpf (protruding-mouth stage). MB, midbrain; PA, pharyngeal arch region; OV, otic vesicle; L, liver; DHB, dorsal hindbrain; NC, notochord.
Fig. 3

The expression of igf2b during early development of zebrafish. (A) Real-time PCR results showing the temporal expression of igf2b relative to a housekeeping gene (18s) in zebrafish embryos during the first 72 hpf; (B–N) Results of whole mount in situ hybridization showing the spatial expression of igf2b during embryogenesis. (B and C) 6 hpf (shield stage), lateral and animal view; (D and E) 12 hpf (somite stage), lateral and animal view; (F and G) 14 hpf (10-somite stage), lateral and animal view; (H and I) 18 hpf (18-somite stage), lateral and animal view; (J) 24 hpf (prim-5 stage), lateral view; (K and L) 48 hpf (long-pec stage), lateral and ventral view. (M and N) 72 hpf (protruding-mouth stage), lateral and dorsal view. FP, floor plate; MB, midbrain; OV, otic vesicle; DHB, dorsal hindbrain; HC, hyperchord; F, fin; B, brain; H, heart.
Fig. 4

The expression of igf3 during early development of zebrafish. (A) Real-time PCR results showing the temporal expression of igf3 relative to a housekeeping gene (18s) in zebrafish embryos during the first 72 hpf; (B–N) Results of whole mount in situ hybridization showing the spatial expression of igf3 during embryogenesis. (B and C) 3 hpf (1 k-cell stage), lateral and animal view; (D and E) 6 hpf (shield stage), lateral and animal view; (F and G) 10 hpf (bud stage), lateral and animal view; (H and I) 18 hpf (18-somite stage), lateral and animal view; (J) 24 hpf (prim-5 stage), lateral view; (K and L) 48 hpf (long-pec stage), lateral and dorsal view. (M and N) 72 hpf (protruding-mouth stage), lateral and dorsal view. PA, pharyngeal arch region; ST, sternohyodieus (hypobranchial muscle).

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Protruding-mouth
Fig. 5

The expression of igf1ra and igf1rb during early development of zebrafish. (A and B) Real-time PCR results showing the temporal expression of igf1ra and igf1rb relative to a housekeeping gene (18s) in zebrafish embryos during the first 72 hpf; (C–G) Results of whole mount in situ hybridization showing the spatial expression of igf1ra during embryogenesis, all are lateral view. (C) 4 hpf (sphere stage); (D) 8 hpf (75% epiboly stage); (E) 10 hpf (bud stage); (F) 24 hpf (prim-5 stage); (G) 72 hpf (protruding-mouth stage). (H–L) Results of whole mount in situ hybridization showing the spatial expression of igf1rb during embryogenesis, all are lateral view. (H) 4 hpf (sphere stage); (I) 8 hpf (75% epiboly stage); (J) 10 hpf (bud stage); (K) 24 hpf (prim-5 stage); (L) 72 hpf (protruding-mouth stage).

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Protruding-mouth
Fig. 7

A schematic diagram of a 72 hpf zebrafish embryo showing the mRNA expression patterns of the four Igf ligands and two Igf type 1 receptors.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene expression patterns : GEP, 15(2), Li, J., Wu, P., Liu, Y., Wang, D., Cheng, C.H., Temporal and Spatial Expression of the four Igf Ligands and two Igf Type 1 Receptors in Zebrafish during Early Embryonic Development, 104-11, Copyright (2014) with permission from Elsevier. Full text @ Gene Expr. Patterns
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