ZFIN ID: ZDB-LAB-050914-1
Gross Lab
PI/Director: Gross, Jeffrey
Contact Person: Gross, Jeffrey
Email: grossjm@pitt.edu
URL: http://www.jeffgrosslab.org
Address: Department of Ophthalmology Louis J. Fox Center for Vision Restoration University of Pittsburgh School of Medicine BST3 Room 2051
Country: United States
Phone: 412-648-2067
Fax:
Line Designation: pt


GENOMIC FEATURES ORIGINATING FROM THIS LAB
Show all 32 genomic features


STATEMENT OF RESEARCH INTERESTS
Our research focuses on vertebrate eye development, disease modeling and regeneration utilizing the zebrafish as a model system. Combining forward genetic screens with reverse genetic and embryological manipulations we hope to understand the molecular, cellular and developmental events that regulate eye formation and visual function, ocular diseases and regenerative responses. Current areas of interest in the lab include studies focusing on the development of the retina, lens and retinal pigmented epithelium (RPE), elucidation of the cellular mechanisms that regulate ocular morphogenesis, and the molecular regulation of retina and RPE regeneration. Our research combines molecular, cellular, biochemical, transgenic and in vivo imaging techniques to address these questions. It is our hope that these studies will ultimately lead to a better understanding of visual system disorders such as macular degeneration, cataracts and ocular colobomata that often result in blindness in afflicted patients, and the development of new therapeutic interventions. Finally, we also have an interest in evolution of the eye and have recently initiated studies in squid to address this topic.


LAB MEMBERS
Hartsock, Andrea Post-Doc Leach, Lyndsay Post-Doc Angileri, Krista Graduate Student
Gath, Natalie Graduate Student Hanovice, Nick Graduate Student Seritrakul, Pawat Graduate Student
Lee, Jiwoon Research Staff


ZEBRAFISH PUBLICATIONS OF LAB MEMBERS
Taler, K., Weiss, O., Rotem, S., Rubinstein, A.M., Seritrakul, P., Gross, J.M., Inbal, A. (2019) Lysyl hydroxylase 3 is required for normal lens capsule formation and maintenance of lens epithelium integrity and fate. Developmental Biology. 458(2):177-188
Seritrakul, P., Gross, J.M. (2019) Genetic and epigenetic control of retinal development in zebrafish. Current opinion in neurobiology. 59:120-127
Gath, N., Gross, J.M. (2019) Zebrafish mab21l2 mutants possess severe defects in optic cup morphogenesis, lens and cornea development. Developmental dynamics : an official publication of the American Association of Anatomists. 248(7):514-529
Hanovice, N.J., Leach, L.L., Slater, K., Gabriel, A.E., Romanovicz, D., Shao, E., Collery, R., Burton, E.A., Lathrop, K.L., Link, B.A., Gross, J.M. (2019) Regeneration of the zebrafish retinal pigment epithelium after widespread genetic ablation. PLoS Genetics. 15:e1007939
Bernstein, C.S., Anderson, M.T., Gohel, C., Slater, K., Gross, J.M., Agarwala, S. (2018) The cellular bases of choroid fissure formation and closure. Developmental Biology. 440:137-151
Siddam, A.D., Gautier-Courteille, C., Perez-Campos, L., Anand, D., Kakrana, A., Dang, C.A., Legagneux, V., Méreau, A., Viet, J., Gross, J.M., Paillard, L., Lachke, S.A. (2018) The RNA-binding protein Celf1 post-transcriptionally regulates p27Kip1 and Dnase2b to control fiber cell nuclear degradation in lens development. PLoS Genetics. 14:e1007278
Hanovice, N.J., McMains, E., Gross, J.M. (2018) Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1821:359-370
Sredni, S.T., Suzuki, M., Yang, J.P., Topczewski, J., Bailey, A.W., Gokirmak, T., Gross, J.N., de Andrade, A., Kondo, A., Piper, D.R., Tomita, T. (2017) A functional screening of the kinome identifies the Polo-like kinase 4 as a potential therapeutic target for malignant rhabdoid tumors, and possibly, other embryonal tumors of the brain. Pediatric blood & cancer. 64(11)
Seritrakul, P., Gross, J.M. (2017) Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways. PLoS Genetics. 13:e1006987
Hanovice, N.J., McMains, E., Gross, J.M. (2017) Corrigendum: A GAL4-inducible transgenic tool kit for the in vivo modulation of Rho GTPase activity in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 246:485
James, A., Lee, C., Williams, A.M., Angileri, K., Lathrop, K.L., Gross, J.M. (2016) The Hyaloid Vasculature Facilitates Basement Membrane Breakdown During Choroid Fissure Closure in the Zebrafish Eye. Developmental Biology. 419(2):262-272
Hanovice, N.J., McMains, E., Gross, J.M. (2016) A GAL4-inducible transgenic toolkit for the in vivo modulation of Rho GTPase activity in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 245(8):844-53
Hanovice, N.J., Daly, C.M., Gross, J.M. (2015) N-Ethylmaleimide-Sensitive Factor b (nsfb) Is Required for Normal Pigmentation of the Zebrafish Retinal Pigment Epithelium. Investigative ophthalmology & visual science. 56:7535-7544
Hartsock, A., Lee, C., Arnold, V., Gross, J.M. (2014) In vivo analysis of Hyaloid vasculature morphogenesis in zebrafish: A role for the lens in maturation and maintenance of the Hyaloid. Developmental Biology. 394(2):327-39
Lee, C., Wallingford, J.B., Gross, J.M. (2014) cluap1 is essential for ciliogenesis and photoreceptor maintenance in the vertebrate eye. Investigative ophthalmology & visual science. 55(7):4585-92
Seritrakul, P., and Gross, J.M. (2014) Expression of the de novo DNA methyltransferases (dnmt3-dnmt8) during zebrafish lens development. Developmental dynamics : an official publication of the American Association of Anatomists. 243(2):350-6
Daly, C.M., Willer, J., Gregg, R., and Gross, J.M. (2013) snow white, a Zebrafish Model of Hermansky-Pudlak Syndrome Type 5. Genetics. 195(2):481-494
Lee, J., Lee, B.K., and Gross, J.M. (2013) Bcl6a function is required during optic cup formation to prevent p53-dependent apoptosis and colobomata. Human molecular genetics. 22(17):3568-82
Lee, J., Cox, B.D., Daly, C.M., Lee, C., Nuckels, R.J., Tittle, R.K., Uribe, R.A., and Gross, J.M. (2012) An ENU Mutagenesis Screen in Zebrafish for Visual System Mutants Identifies a Novel Splice-Acceptor Site Mutation in patched2 that Results in Colobomas. Investigative ophthalmology & visual science. 53(13):8214-8221
Hayes, J.M., Hartsock, A., Clark, B.S., Napier, H.R., Link, B.A., and Gross, J.M. (2012) Integrin α5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish. Molecular biology of the cell. 23(24):4725-4738
Uribe, R.A., Kwon, T., Marcotte, E.M., and Gross, J.M. (2012) Id2a functions to limit Notch pathway activity and thereby influence the transition from proliferation to differentiation of retinoblasts during zebrafish retinogenesis. Developmental Biology. 371(2):280-292
Luo, J., Uribe, R.A., Hayton, S., Calinescu, A.A., Gross, J.M., and Hitchcock, P.F. (2012) Midkine-A functions upstream of Id2a to regulate cell cycle kinetics in the developing vertebrate retina. Neural Development. 7(1):33
Bibliowicz, J., and Gross, J.M. (2011) Ectopic proliferation contributes to retinal dysplasia in the juvenile zebrafish patched2 mutant eye. Investigative ophthalmology & visual science. 52(12):8868-77
Tittle, R.K., Sze, R., Ng, A., Nuckels, R.J., Swartz, M.E., Anderson, R.M., Bosch, J., Stainier, D.Y., Eberhart, J.K., and Gross, J.M. (2011) Uhrf1 and Dnmt1 are required for development and maintenance of the zebrafish lens. Developmental Biology. 350(1):50-63
Bibliowicz, J., Tittle, R.K., and Gross, J.M. (2011) Toward a Better Understanding of Human Eye Disease Insights From the Zebrafish, Danio rerio. Progress in molecular biology and translational science. 100:287-300
Uribe, R.A., and Gross, J.M. (2010) Id2a influences neuron and glia formation in the zebrafish retina by modulating retinoblast cell cycle kinetics. Development (Cambridge, England). 137(22):3763-3774
Bibliowicz, J., and Gross, J.M. (2009) Expanded progenitor populations, vitreo-retinal abnormalities, and Muller glial reactivity in the zebrafish leprechaun/patched2 retina. BMC Developmental Biology. 9:52
Ng, A., Uribe, R.A., Yieh, L., Nuckels, R., and Gross, J.M. (2009) Zebrafish mutations in gart and paics identify crucial roles for de novo purine synthesis in vertebrate pigmentation and ocular development. Development (Cambridge, England). 136(15):2601-2611
Brown, J.D., Dutta, S., Bharti, K., Bonner, R.F., Munson, P.J., Dawid, I.B., Akhtar, A.L., Onojafe, I.F., Alur, R.P., Gross, J.M., Hejtmancik, J.F., Jiao, X., Chan, W.Y., and Brooks, B.P. (2009) Expression profiling during ocular development identifies 2 Nlz genes with a critical role in optic fissure closure. Proceedings of the National Academy of Sciences of the United States of America. 106(5):1462-1467
Nuckels, R.J., Ng, A., Darland, T., and Gross, J.M. (2009) The Vacuolar-ATPase Complex Regulates Retinoblast Proliferation and Survival, Photoreceptor Morphogenesis and Pigmentation in the Zebrafish Eye. Investigative ophthalmology & visual science. 50(2):893-905
Lee, J., Willer, J.R., Willer, G.B., Smith, K., Gregg, R.G., and Gross, J.M. (2008) Zebrafish blowout provides genetic evidence for Patched1-mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye. Developmental Biology. 319(1):10-22
Gross, J.M., and Perkins, B.D. (2008) Zebrafish mutants as models for congenital ocular disorders in humans. Molecular reproduction and development. 75(3):547-555
Lee, J., and Gross, J.M. (2007) Laminin {beta}1 and {gamma}1 Containing Laminins Are Essential for Basement Membrane Integrity in the Zebrafish Eye. Investigative ophthalmology & visual science. 48(6):2483-2490
Gross, J.M., Perkins, B.D., Amsterdam, A., Egana, A., Darland, T., Matsui, J.I., Sciascia, S., Hopkins, N., and Dowling, J.E. (2005) Identification of Zebrafish Insertional Mutants with Defects in Visual System Development and Function. Genetics. 170(1):245-261
Gross, J.M., and Dowling, J.E. (2005) Tbx2b is essential for neuronal differentiation along the dorsal/ventral axis of the zebrafish retina. Proceedings of the National Academy of Sciences of the United States of America. 102(12):4371-4376