PUBLICATION
Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish
- Authors
- Hanovice, N.J., McMains, E., Gross, J.M.
- ID
- ZDB-PUB-180801-12
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1821: 359-370 (Chapter)
- Registered Authors
- Gross, Jeffrey, Hanovice, Nick
- Keywords
- Cdc42, GAL4/UAS, Heat shock, Rac1, RhoA, Transgenics, Zebrafish
- MeSH Terms
-
- rho GTP-Binding Proteins*/biosynthesis
- rho GTP-Binding Proteins*/genetics
- Embryo, Nonmammalian/embryology*
- Transcription Factors*/genetics
- Transcription Factors*/metabolism
- Gene Expression Regulation, Developmental
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Gene Expression Regulation, Enzymologic
- Embryonic Development*
- Animals
- Animals, Genetically Modified*/embryology
- Animals, Genetically Modified*/genetics
- DNA-Binding Proteins*/genetics
- DNA-Binding Proteins*/metabolism
- PubMed
- 30062424 Full text @ Meth. Mol. Biol.
Citation
Hanovice, N.J., McMains, E., Gross, J.M. (2018) Using GAL4-Inducible Transgenics to Modulate Rho GTPase Activity in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1821:359-370.
Abstract
Rho GTPases are Ras-family G proteins that regulate many critical cellular functions. Due to their requirement during early embryonic development, investigations into the function of Rho GTPases at a tissue-specific level require inducible and spatially targeted modulation of Rho GTPase activity. Here, we describe the use of ten novel zebrafish transgenics enabling GAL4-specific expression of Rho GTPases to modulate Rho GTPase activity with spatial and temporal control.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping