Gross, J.M., Perkins, B.D., Amsterdam, A., Egana, A., Darland, T., Matsui, J.I., Sciascia, S., Hopkins, N., and Dowling, J.E. (2005) Identification of Zebrafish Insertional Mutants with Defects in Visual System Development and Function. Genetics. 170(1):245-261.
Genetic analysis in zebrafish has been instrumental in identifying genes necessary for visual system development and function. Recently, a large-scale retroviral insertional mutagenesis screen was completed in which 315 different genes were mutated that resulted in obvious phenotypic defects by 5 days post fertilization. That the disrupted gene has been identified in each of these mutants provides a unique resource through which the formation, function or physiology of individual organ systems can be studied. To that end, a screen for visual system mutants was performed on 250 of the mutants in this collection examining each of them histologically for morphological defects in the eye, and behaviorally for overall visual system function. Forty loci were identified whose disruption resulted in defects in eye development and/or visual function. The mutants have been divided into the following phenotypic classes that show defects in: (1) morphogenesis; (2) growth and central retinal development; (3) the peripheral marginal zone; (4) retinal lamination; (5) the photoreceptor cell layer; (6) the retinal pigment epithelium; (7) the lens; (8) retinal containment and (9) behavior. The affected genes in these mutants highlight a diverse set of proteins necessary for the development, maintenance and function of the vertebrate visual system.