Gene
rfx2
- ID
- ZDB-GENE-050227-4
- Name
- regulatory factor X, 2 (influences HLA class II expression)
- Symbol
- rfx2 Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 8 Mapping Details/Browsers
- Description
- Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within Kupffer's vesicle development and determination of left/right symmetry. Predicted to be located in cytoplasm and nucleus. Is expressed in several structures, including central nervous system; mesoderm; neural plate; neural tube; and pronephros. Orthologous to human RFX2 (regulatory factor X2).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 38 figures from 13 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cb7 (16 images)
- cb133 (8 images)
Wild Type Expression Summary
- All Phenotype Data
- 3 figures from Bisgrove et al., 2012
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| ct81Gt | Transgenic insertion | Unknown | Unknown | DNA | |
| la010683Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| la016289Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| la026392Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| sa14019 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa21260 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa31644 | Allele with one point mutation | Unknown | Premature Stop | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| MO1-rfx2 | N/A | (3) |
| MO2-rfx2 | N/A | Bisgrove et al., 2012 |
| MO3-rfx2 | N/A | Bisgrove et al., 2012 |
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Human Disease
Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Domain | IPR003150 | DNA-binding RFX-type winged-helix domain |
| Domain | IPR007668 | RFX1 transcription activation region |
| Family | IPR039779 | RFX-like DNA-binding protein |
| Homologous_superfamily | IPR036388 | Winged helix-like DNA-binding domain superfamily |
| Homologous_superfamily | IPR036390 | Winged helix DNA-binding domain superfamily |
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Domain Details Per Protein
| Protein | Length | DNA-binding RFX-type winged-helix domain | RFX1 transcription activation region | RFX-like DNA-binding protein | Winged helix DNA-binding domain superfamily | Winged helix-like DNA-binding domain superfamily |
|---|---|---|---|---|---|---|
|
UniProtKB:Q5EAP5
|
734 | |||||
|
UniProtKB:A0A8M2BEA3
|
704 | |||||
|
UniProtKB:A0A8M2BEC2
|
709 |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(hsp70l:rfx2-HA) |
|
| 1 | (2) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-275D22 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-240A9 | ZFIN Curated Data | |
| Encodes | EST | cb7 | ||
| Encodes | EST | cb133 | Thisse et al., 2001 | |
| Encodes | EST | fd20b11 | ||
| Encodes | cDNA | MGC:91808 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001013278 (1) | 3689 nt | ||
| Genomic | GenBank:BX664738 (2) | 180024 nt | ||
| Polypeptide | UniProtKB:Q5EAP5 (1) | 734 aa |
- Xie, H., Kang, Y., Wang, S., Zheng, P., Chen, Z., Roy, S., Zhao, C. (2020) E2f5 is a versatile transcriptional activator required for spermatogenesis and multiciliated cell differentiation in zebrafish. PLoS Genetics. 16:e1008655
- Zhang, J., Chandrasekaran, G., Li, W., Kim, D.Y., Jeong, I.Y., Lee, S.H., Liang, T., Bae, J.Y., Choi, I., Kang, H., Maeng, J.S., Kim, M.K., Lee, T., Park, S.W., Kim, M.J., Kim, H.S., Ro, H., Bae, Y.C., Park, H.C., Choi, E.Y., Choi, S.Y. (2020) Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord. Nature communications. 11:1860
- Tavares, B., Jacinto, R., Sampaio, P., Pestana, S., Pinto, A., Vaz, A., Roxo-Rosa, M., Gardner, R., Lopes, T., Schilling, B., Henry, I., Saúde, L., Lopes, S.S. (2017) Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer.. eLIFE. 6
- Jaffe, K.M., Grimes, D.T., Schottenfeld-Roames, J., Werner, M.E., Ku, T.J., Kim, S.K., Pelliccia, J.L., Morante, N.F., Mitchell, B.J., Burdine, R.D. (2016) c21orf59/kurly Controls Both Cilia Motility and Polarization. Cell Reports. 14(8):1841-9
- Shim, H., Kim, J.H., Kim, C.Y., Hwang, S., Kim, H., Yang, S., Lee, J.E., Lee, I. (2016) Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource. Nucleic acids research. 44:9611-9623
- Elkon, R., Milon, B., Morrison, L., Shah, M., Vijayakumar, S., Racherla, M., Leitch, C.C., Silipino, L., Hadi, S., Weiss-Gayet, M., Barras, E., Schmid, C.D., Ait-Lounis, A., Barnes, A., Song, Y., Eisenman, D.J., Eliyahu, E., Frolenkov, G.I., Strome, S.E., Durand, B., Zaghloul, N.A., Jones, S.M., Reith, W., Hertzano, R. (2015) RFX transcription factors are essential for hearing in mice. Nature communications. 6:8549
- Liu, J., Merkle, F.T., Gandhi, A.V., Gagnon, J.A., Woods, I.G., Chiu, C.N., Shimogori, T., Schier, A.F., Prober, D.A. (2015) Evolutionarily conserved regulation of hypocretin neuron specification by Lhx9. Development (Cambridge, England). 142(6):1113-24
- Lu, H., Toh, M.T., Narasimhan, V., Thamilselvam, S.K., Choksi, S.P., Roy, S. (2015) A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation. Developmental Biology. 397(2):225-36
- Verleyen, D., Luyten, F.P., Tylzanowski, P. (2014) Orphan G-Protein Coupled Receptor 22 (Gpr22) Regulates Cilia Length and Structure in the Zebrafish Kupffer's Vesicle. PLoS One. 9:e110484
- Arrington, C.B., Peterson, A.G., and Yost, H.J. (2013) Sdc2 and Tbx16 regulate Fgf2-dependent epithelial cell morphogenesis in the ciliated organ of asymmetry. Development (Cambridge, England). 140(19):4102-4109
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