ZFIN ID: ZDB-PUB-010810-1
Expression of the zebrafish genome during embryogenesis (NIH R01 RR15402)
Thisse, B., Pflumio, S., Fürthauer, M., Loppin, B., Heyer, V., Degrave, A., Woehl, R., Lux, A., Steffan, T., Charbonnier, X.Q. and Thisse, C.
Date: 2001
Source: ZFIN Direct Data Submission: (Unpublished)
Registered Authors: Degrave, Agnes, Fürthauer, Maximilian, Heyer, Vincent, Loppin, Benjamin, Obrecht-Pflumio, Sophie, Steffan, Tania, Thisse, Bernard, Thisse, Christine, Woehl, Roxane
Keywords: none
MeSH Terms: none
PubMed: none
ABSTRACT

Summary
We perform a large scale in situ hybridization screen to characterize genes expressed in a spatially regulated manner during zebrafish embryogenesis. cDNAs collected from various zebrafish cDNA libraries are used as templates for the synthesis of digoxygenin labeled antisense RNA probes. These probes are subsequently used to analyze the expression pattern of the corresponding gene at different developmental stages (from gastrula to hatching). cDNAs associated with a spatially restricted expression pattern are sequenced at their 5' and 3' ends to provide information about the encoded protein product and to map the corresponding gene genetically. For each clone analyzed, we provide 5' and 3' sequences of the cDNA (deposited in Genbank), the result of BLAST analysis (against protein databases: SwissProt and translated EMBL) as well as a description of the corresponding expression pattern at six different stages (gastrula, early somitogenesis, middle of somitogenesis, 24h, 36h and 48h) and a set of annotated pictures. These data are deposited in the ZFIN database and cDNA clones are publically available upon request at the Zebrafish International Resource Center . This work provides hundreds of specific cell or tissue markers to analyze mutant phenotypes and to help identify candidates for mutant loci or downstream targets of regulatory genes. This project allows the description of zebrafish embryonic development in terms of gene expression and will eventually establish a "molecular anatomy" of the developing embryo.

Method:

Acknowledging this work:
All clones, information, and images used from this work should cite this summary and first description of this work posted in ZFIN: (Thisse, B, Pfumio, S., Fürthauer, M., Loppin B., Heyer, V., Degrave, A., Woehl, R., Lux, A., Steffan, T., Charbonnier, X.Q. and Thisse, C. Expression of the zebrafish genome during embryogenesis. ZFIN on-line publication, 2001). This work is supported by grant RR15402-01 from the NIH. Please acknowledge this grant number in all publications resulting from the materials we are providing to you. If you have any questions or comments on this project, please contact CB Thisse.

ADDITIONAL INFORMATIONNo data available