Liu et al., 2003 - Fgf3 and Fgf8 dependent and independent transcription factors are required for otic placode specification. Development (Cambridge, England)   130(10):2213-2224 Full text @ Development

Fig. 5

sox9b functions in otic specification. (A,B) The splice-blocking morpholino against sox9b (sox9bMO) significantly reduces the size of the otic vesicle (B, n=40 embryos), compared with that of wild-type control embryos (A). (C,D) In jellyfish (jef) mutants that harbor a retroviral insertion in the sox9a gene (jefhil134) (Amsterdam et al., 1999; Yan et al., 2002), injecting sox9b-MOs results in the loss of a morphologically recognizable otic vesicle (n=9 embryos), although a few dispersed pax2a-positive cells persist (D). In uninjected jef (sox9a) mutants (C, n=6 embryos), otic vesicles are slightly smaller than in wild-type control embryos (A). (E,F) Injecting sox9b-MO blocks formation of the residual otic cells of Dfb380 (4/6 Dfb380 embryos had residual pax2a-expressing cells, only 2/12 Dfb380 embryos had residual otic cells after injection with sox9b-MO). (A,B,E,F) prim-12 (28 h); (D,F) prim 25 (36 h). Side views, anterior towards the left, dorsal towards the top. Scale bar: 25 μm.

Fish: AB + MO1-sox9b + MO2-sox9b, sox9ahi1134Tg + MO1-sox9b + MO2-sox9b
Knockdown Reagents: MO1-sox9b, MO2-sox9b
Observed In: otic vesicle
Stage Range: Prim-5 to Prim-25

This is a summary of gene expression assays reported in this publication. Associated figures and anatomical structures have not yet been added to ZFIN.

Genes: cldna, dlx3b, dlx4b, egr2b, fn1a (all 8) expand
Fish: WT, sox9ahi1134Tg, Df(Chr12:dlx3b,dlx4b,tbx6)b380/b380
Anatomical Term: unspecified
Stage Range: 5-9 somites to Prim-25
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development