Liu et al., 2003 - Fgf3 and Fgf8 dependent and independent transcription factors are required for otic placode specification. Development (Cambridge, England)   130(10):2213-2224 Full text @ Development

Fig. 5

sox9b functions in otic specification. (A,B) The splice-blocking morpholino against sox9b (sox9bMO) significantly reduces the size of the otic vesicle (B, n=40 embryos), compared with that of wild-type control embryos (A). (C,D) In jellyfish (jef) mutants that harbor a retroviral insertion in the sox9a gene (jefhil134) (Amsterdam et al., 1999; Yan et al., 2002), injecting sox9b-MOs results in the loss of a morphologically recognizable otic vesicle (n=9 embryos), although a few dispersed pax2a-positive cells persist (D). In uninjected jef (sox9a) mutants (C, n=6 embryos), otic vesicles are slightly smaller than in wild-type control embryos (A). (E,F) Injecting sox9b-MO blocks formation of the residual otic cells of Dfb380 (4/6 Dfb380 embryos had residual pax2a-expressing cells, only 2/12 Dfb380 embryos had residual otic cells after injection with sox9b-MO). (A,B,E,F) prim-12 (28 h); (D,F) prim 25 (36 h). Side views, anterior towards the left, dorsal towards the top. Scale bar: 25 μm.

Unillustrated author statements

ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development