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FIGURE 1

Oligo‐fucoidan pre‐treatment reduced the expression of lipogenesis genes, fibrosis marker and decreased the levels of collagen fibers in adult [HBx,src] transgenic zebrafish. A, Expression of lipogenic factors (pparg, mlxip, and srebf1), B, lipogenic enzymes (agpat4, pap and fasn), and C, fibrosis markers (ctgfa, hpse, and col1a1a). Data are presented as dot plots, each dots represent one fish. Statistical significance was calculated by t‐test (*P ≤ .05, **P ≤ .01, ***P ≤ .001). D, Representative images and statistical analysis of Sirius Red stain in [HBx,src] transgenic fish. Scale bar represents 50 μm. WT represents non‐transgenic wild‐type control fish, WT+OF stands for WT fish feeding with OF, NOR indicates normal diet, DIO denotes diet‐induced obesity, DIO+OF represents DIO fish given oral gavage with 0.051 mg OF.

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