FIGURE 6

Loss of tet2 associated with changes in expression of genes associated with inflammation, metabolism, and the cell cycle in 4-month HSPCs. (A) Volcano plot representing the differentially expressed genes identified from the bulk-RNAseq sequencing data. Differentially expressed genes were identified between 4-month-old tet2^m/m and 4-month-old tet2^wt/wt HSPCs, with 4-month-old tet2^wt/wt as the baseline. The genes with an absolute log2-fold change greater than 1 and an adjusted p-value less than 0.05 are colored. The upregulated genes in the 4-month-old tet2^m/m fish are colored in violet and the downregulated genes in the 4-month-old tet2^wt/wt fish are colored in dark green. (B) Pathway enrichment analysis of the upregulated genes against the hallmark database was conducted using EnrichR. The top five significant pathways (p < 0.05) were plotted. The x-axis indicates the negative logarithm of the p-value from the enrichment results. The y-axis represents individual pathways. (C) Pathway enrichment analysis of the downregulated genes against the hallmark database was conducted using EnrichR. The top five significant pathways were plotted. The x-axis indicates the negative logarithm of the p-value from the enrichment results. The y-axis represents individual pathways.