FIGURE

Fig. 5

ID

ZDB-FIG-230809-11

Publication

Prendergast et al., 2023 - CSF-contacting neurons respond to Streptococcus pneumoniae and promote host survival during central nervous system infection

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Fig. 5

Figure 5. RNA-seq shows upregulation of complement system of the alternative and terminal pathway as well as cytokines in CSF-cNs during S. pneumoniae infection

(A) Log fold change bar plot comparing enrichment of known CSF-cN transcripts in the GFP⁺ fraction with control GFP⁻ cells in the original differential transcriptome 1 (gray bars; see Figure 4 and Table S1) and subsequent transcriptome 2 for non-infected larvae (black bars; see Table S1).

(B) A plot of raw reads comparing selected receptors in non-infected (gray bars) and infected (pink bars) CSF-cNs, showing that the expression of receptors that are specifically enriched in CSF-cNs (pkd2l1, pkd1l2, and taste receptors) remains stable.

(C) Volcano plot comparing the expression of transcripts in infected CSF-cNs relative to uninfected CSF-cNs. Transcripts for several immune-related secretory compounds are differentially expressed.

(D) Log fold change bar plot of selected immune-related transcripts corresponding to complement system and cytokines from the same data as in (B). We observe significant upregulation of complement system of the alternative (C3), classical (C4), and terminal (C6) pathway (left side) as well as cytokines (right side).