FIGURE

Fig. 3

ID
ZDB-FIG-230728-20
Publication
Lin et al., 2023 - YULINK regulates vascular formation in zebrafish and HUVECs
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

FLIM-FRET analysis of proteins interacting with YULINK. A Plasmid construct for full-length YULINK protein containing four conserved WD40 repeats (blue) and 3 potential WD40 candidates (purple). Other plasmid constructs for truncated forms of N- or C-termini (YULINK∆N or YULINK∆C) are also shown. BD To validate these interactions, YULINK (full-length or truncated forms) was conjugated with AcGFP and the interacting protein candidates, EPS15, RAB33B, or TICAM2, were conjugated with DsRed, separately. Then, they were co-expressed in HEK-293 T cells and the FRET (Förster Resonance Energy Transfer) of AcGFP was measured using multi-photon fluorescence lifetime imaging microscopy (FLIM). The mean fluorescence lifetime (τ) and the FRET efficiency E (%) were measured at 48 h after co-transfection of HEK-293 T cells with AcGFP-YULINK (full-length or truncated forms) and DsRed-EPS15, DsRed-RAB33B, or DsRed-TICAM2, respectively. The less mean fluorescence lifetime (τ) and more FRET efficiency E (%) indicated stronger interaction and shorter distance between AcGFP and DsRed. The fluorescence lifetime (τ) of cells expressing only AcGFP-YULINK was as a FLIM-FRET control

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Res.