PUBLICATION
YULINK regulates vascular formation in zebrafish and HUVECs
- Authors
- Lin, H.H., Kuo, M.W., Fan, T.C., Yu, A.L., Yu, J.
- ID
- ZDB-PUB-230228-31
- Date
- 2023
- Source
- Biological research 56: 77 (Journal)
- Registered Authors
- Keywords
- Clathrin, EPS15, Endocytosis, RAB33B, RHOB, TICAM2, VEGF, VEGFR2, Vasculogenesis, YULINK
- MeSH Terms
-
- Animals
- Cell Differentiation
- Cell Movement
- Human Umbilical Vein Endothelial Cells
- Humans
- Neovascularization, Physiologic
- Saccharomyces cerevisiae*
- Zebrafish*/genetics
- PubMed
- 36843032 Full text @ Biol. Res.
Citation
Lin, H.H., Kuo, M.W., Fan, T.C., Yu, A.L., Yu, J. (2023) YULINK regulates vascular formation in zebrafish and HUVECs. Biological research. 56:77.
Abstract
Background The distinct arterial and venous cell fates are dictated by a combination of various genetic factors which form diverse types of blood vessels such as arteries, veins, and capillaries. We report here that YULINK protein is involved in vasculogenesis, especially venous formation.
Methods In this manuscript, we employed gene knockdown, yeast two-hybrid, FLIM-FRET, immunoprecipitation, and various imaging technologies to investigate the role of YULINK gene in zebrafish and human umbilical vein endothelial cells (HUVECs).
Results Knockdown of YULINK during the arterial-venous developmental stage of zebrafish embryos led to the defective venous formation and abnormal vascular plexus formation. Knockdown of YULINK in HUVECs impaired their ability to undergo cell migration and differentiation into a capillary-like tube formation. In addition, the phosphorylated EPHB4 was decreased in YULINK knockdown HUVECs. Yeast two-hybrid, FLIM-FRET, immunoprecipitation, as well as imaging technologies showed that YULINK colocalized with endosome related proteins (EPS15, RAB33B or TICAM2) and markers (Clathrin and RHOB). VEGF-induced VEGFR2 internalization was also compromised in YULINK knockdown HUVECs, demonstrating to the involvement of YULINK.
Conclusion This study suggests that YULINK regulates vasculogenesis, possibly through endocytosis in zebrafish and HUVECs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping