FIGURE

Fig. 2

ID

ZDB-FIG-220627-36

Publication

Wang et al., 2022 - α-Ketoglutarate-Mediated DNA Demethylation Sustains T-Acute Lymphoblastic Leukemia upon TCA Cycle Targeting

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

DLST inactivation increases transcript and protein levels of IDH1 and IDH2 in human T-ALL cells. (A,B) qRT-PCR analysis of IDH1 (A) and IDH2 (B) transcript levels at 4 days transduction of shLuciferase and shDLST in human MOLT3, PEER, and JURKAT T-ALL cell lines. Relative fold change was analyzed using the shLuciferase group as a control. The data are presented as the mean ± s.e.m, and statistical differences were calculated using one-way ANOVA. (C) Western blotting of DLST, IDH1, IDH2, and LDHA in the same three T-ALL cell lines transduced with shLuciferase or shDLST hairpins. Relative protein to ACTIN ratios are shown at the bottom of each western blotting panel. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancers