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Fig. 2

ID
ZDB-IMAGE-220627-26
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Figures for Wang et al., 2022
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Figure Caption

Fig. 2

DLST inactivation increases transcript and protein levels of IDH1 and IDH2 in human T-ALL cells. (A,B) qRT-PCR analysis of IDH1 (A) and IDH2 (B) transcript levels at 4 days transduction of shLuciferase and shDLST in human MOLT3, PEER, and JURKAT T-ALL cell lines. Relative fold change was analyzed using the shLuciferase group as a control. The data are presented as the mean ± s.e.m, and statistical differences were calculated using one-way ANOVA. (C) Western blotting of DLST, IDH1, IDH2, and LDHA in the same three T-ALL cell lines transduced with shLuciferase or shDLST hairpins. Relative protein to ACTIN ratios are shown at the bottom of each western blotting panel. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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