FIGURE

Fig. 1

ID

ZDB-FIG-220627-35

Publication

Wang et al., 2022 - α-Ketoglutarate-Mediated DNA Demethylation Sustains T-Acute Lymphoblastic Leukemia upon TCA Cycle Targeting

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Fig. 1

DLST inactivation increased reductive carboxylation while reducing TCA cycle intermediates in human T-ALL cells. (A) Scheme of ¹³C labeled glutamine contribution to TCA cycle metabolites. (B) Percentage of ¹³C-labelled glutamine uptake in human MOLT3 cells in the presence or absence of DLST knockdown. The insert in panel (B) shows efficient depletion of DLST by shDLST1 hairpin. (C–H) Percentages of TCA cycle metabolites derived from ¹³C labeled glutamine in the presence or absence of DLST knockdown. M stands for mass. The labels for M + 0, M + 1, M + 2, M + 3, M + 4, and M + 5 indicate that no or one to five carbons in the metabolite are derived from ¹³C labeled glutamine. The data are presented as the mean ± s.e.m. and analyzed with an unpaired two-tailed t-test for statistical significance. * p < 0.05, ** p < 0.01, *** p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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