FIGURE

Figure 7

ID

ZDB-FIG-210902-191

Publication

Song et al., 2021 - Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models

Other Figures

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Figure 7

Generation of the slc16a2 mutant zebrafish. (A) Schematic illustration representing the zebrafish Slc16a2 protein (upper panel) and slc16a2 locus with the CRISPR target site on exon 1 (middle panel). Sequencing of the slc16a2 target CRISPR site in wild type, slc16a2 (-28) mutant, and slc16a2 (-10) mutant zebrafish lines are shown in the lower panel. (B) The predicted truncated Slc16a2 proteins resulting from premature translational termination are shown. (C) Quantitative RT-PCR analyses of tshba, tg, tpo, and slc5a5 expression in slc16a2 mutants and their wild-type siblings at 5 dpf. (D) Quantitative RT-PCR analyses of slc16a2, slc16a10, and slco1c1 expressions in slc16a2 mutants and their wild-type siblings at 5 dpf. (E) Whole-body T4 contents of slc16a2 mutants and their wild-type siblings at 20, 25, and 60 dpf. (F) Whole-body T3 contents of slc16a2 mutants and their wild-type siblings at 20, 25, and 60 dpf. ** p < 0.01, ns: No significance.

Expression Data

Genes:	slc5a5 slc16a2 slc16a10 slco1c1 tg tpo tshba
Fish:	AB slc16a2^{ihb566/ihb566} (AB)
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc16a2^{ihb566/ihb566} (AB)
Observed In:	whole organism whole organism 3,3',5-triiodo-L-thyronine whole organism thyroxine
Stage Range:	Day 5 to Days 45-89

Phenotype Detail

Acknowledgments

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