Figure 5

Changes in secondary sex characteristics in tshba mutant zebrafish. (A,B) Representative histological sections of ovaries from wild-type zebrafish at 4 mpf (A) and from tshba mutant zebrafish at 8 mpf (B) by H&E staining. (C,D) Representative histological sections of testes from wild-type zebrafish at 4 mpf (C) and from tshba mutant zebrafish at 8 mpf (D) by H&E staining. (E,H,K,N) Gross morphology of wild-type female fish at 4 mpf (E), tshba mutant female fish at 8 mpf (H), wild-type male fish at 4 mpf (K), and tshba mutant male fish at 8 mpf (N). (F,I) Representative female vent in wild-type (F) and tshba mutant (I) fish. Red asterisks indicate the vent anterior to the anal fin. (L,O) Representative male vent in the wild-type (L) and tshba mutant (O) fish. (G,J,M,P) Pectoral fins of wild-type female fish (G), tshba mutant female fish (J), wild-type male fish (M), and tshba mutant male fish (P) are shown. Representative breeding tubercles (BTs) were seen in wild-type male fish (M). No BTs were seen in tshba mutant male fish (L). Red arrowheads indicate the BTs on the pectoral fins.