ZFIN ID: ZDB-FIG-200314-57
Rojas-Benítez et al., 2020 - Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development. International Journal of Molecular Sciences   21(3) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Figure 4

Shh activity is diminished in ELP morphants. (A) Immunohistochemistry usnig an anti-Engrailed antibody to visualize the adaxial cells. Control, elp3 morphants and MO/PKADN mRNA-injected animals were analyzed 24 hpf. (B) The number of engrailed(+) cells were counted (N = 4, n = 30, ANOVA *** p < 0.005). (C) In situ hybridization to detect the expression pattern of col2a1 in control, elp3 morphants and MO/PKADN mRNA-injected fish (images representative of four independent experiments). Scale bar 40 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a WT control Prim-5 notochord ISH
WT + MO1-elp3 standard conditions Prim-5 notochord ISH
WT + MO2-elp3 standard conditions Prim-5 notochord ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC WT control Prim-5 adaxial cell
IHC WT + MO1-elp3 standard conditions Prim-5 adaxial cell
IHC WT + MO2-elp3 standard conditions Prim-5 adaxial cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-elp3 standard conditions Prim-5 adaxial cell ab1-eng labeling decreased amount, abnormal
Prim-5 notochord col2a1a expression decreased amount, abnormal
WT + MO2-elp3 standard conditions Prim-5 adaxial cell ab1-eng labeling decreased amount, abnormal
Prim-5 notochord col2a1a expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.