ZFIN Figure: Rojas-Benítez et al., 2020, Figure 4

ZFIN ID: ZDB-FIG-200314-57

ZFIN ID: ZDB-FIG-200314-57

Rojas-Benítez et al., 2020 - Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development. International Journal of Molecular Sciences 21(3) Full text @ Int. J. Mol. Sci.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	col2a1a
Antibody:	Ab1-eng
Fish:	WT WT + MO1-elp3 WT + MO2-elp3
Knockdown Reagents:	MO1-elp3 MO2-elp3
Anatomical Terms:	adaxial cell notochord
Stage:	Prim-5

PHENOTYPE:

Fish:	WT + MO1-elp3 WT + MO2-elp3
Knockdown Reagents:	MO1-elp3 MO2-elp3
Observed In:	adaxial cell notochord
Stage:	Prim-5

Figure 4

Shh activity is diminished in ELP morphants. (A) Immunohistochemistry usnig an anti-Engrailed antibody to visualize the adaxial cells. Control, elp3 morphants and MO/PKA^DN mRNA-injected animals were analyzed 24 hpf. (B) The number of engrailed(+) cells were counted (N = 4, n = 30, ANOVA *** p < 0.005). (C) In situ hybridization to detect the expression pattern of col2a1 in control, elp3 morphants and MO/PKA^DN mRNA-injected fish (images representative of four independent experiments). Scale bar 40 µm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
col2a1a	WT	control	Prim-5	notochord	ISH
	WT + MO1-elp3	standard conditions	Prim-5	notochord	ISH
	WT + MO2-elp3	standard conditions	Prim-5	notochord	ISH

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab1-eng	IHC	WT	control	Prim-5	adaxial cell
	IHC	WT + MO1-elp3	standard conditions	Prim-5	adaxial cell
	IHC	WT + MO2-elp3	standard conditions	Prim-5	adaxial cell

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO1-elp3	standard conditions	Prim-5	adaxial cell ab1-eng labeling decreased amount, abnormal
		Prim-5	notochord col2a1a expression decreased amount, abnormal
WT + MO2-elp3	standard conditions	Prim-5	adaxial cell ab1-eng labeling decreased amount, abnormal
		Prim-5	notochord col2a1a expression decreased amount, abnormal

Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.