Shh activity is diminished in ELP morphants. (A) Immunohistochemistry usnig an anti-Engrailed antibody to visualize the adaxial cells. Control, elp3 morphants and MO/PKADN mRNA-injected animals were analyzed 24 hpf. (B) The number of engrailed(+) cells were counted (N = 4, n = 30, ANOVA *** p < 0.005). (C) In situ hybridization to detect the expression pattern of col2a1 in control, elp3 morphants and MO/PKADN mRNA-injected fish (images representative of four independent experiments). Scale bar 40 µm.