ZFIN Figure: Rojas-Benítez et al., 2020, Figure 3

ZFIN ID: ZDB-FIG-200314-56

ZFIN ID: ZDB-FIG-200314-56

Rojas-Benítez et al., 2020 - Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development. International Journal of Molecular Sciences 21(3) Full text @ Int. J. Mol. Sci.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Antibodies:	Ab-F59 Ab-S58
Fish:	WT WT + MO1-elp3 WT + MO2-elp3
Knockdown Reagents:	MO1-elp3 MO2-elp3
Anatomical Terms:	fast muscle cell slow muscle cell
Stage:	Long-pec

PHENOTYPE:

Fish:	WT + MO1-elp3 WT + MO2-elp3
Knockdown Reagents:	MO1-elp3 MO2-elp3
Observed In:	somite fast muscle cell somite slow muscle cell
Stage:	Long-pec

Figure 3

Abnormal muscle fiber morphology in morphants. Muscle fibers of control (A, D, G and J), morphants (B, E, H and K) and MO + PKADN mRNA injected (C, F, I and L) were analyzed. Upper panels show fast fibers (F59) and lower show slow fibers (S58). F-actin was stained with TRICT-phalloidin. Scale bar 50 µm. Images representative of 4 independent experiments.

Gene Expression Details No data available

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab-F59	IHC	WT	control	Long-pec	fast muscle cell
	IHC	WT + MO1-elp3	standard conditions	Long-pec	fast muscle cell
	IHC	WT + MO2-elp3	standard conditions	Long-pec	fast muscle cell
Ab-S58	IHC	WT	control	Long-pec	slow muscle cell
	IHC	WT + MO1-elp3	standard conditions	Long-pec	slow muscle cell
	IHC	WT + MO2-elp3	standard conditions	Long-pec	slow muscle cell

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO1-elp3	standard conditions	Long-pec	somite fast muscle cell disorganized, abnormal
		Long-pec	somite slow muscle cell morphology, abnormal
		Long-pec	somite slow muscle cell undulate, abnormal
WT + MO2-elp3	standard conditions	Long-pec	somite fast muscle cell disorganized, abnormal
		Long-pec	somite slow muscle cell morphology, abnormal
		Long-pec	somite slow muscle cell undulate, abnormal

Acknowledgments:
ZFIN wishes to thank the journal International Journal of Molecular Sciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Int. J. Mol. Sci.