PUBLICATION
Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
- Authors
- Rojas-Benítez, D., L Allende, M.
- ID
- ZDB-PUB-200207-22
- Date
- 2020
- Source
- International Journal of Molecular Sciences 21(3): (Journal)
- Registered Authors
- Keywords
- elongator complex, tRNA modification, zebrafish
- MeSH Terms
-
- Animals
- Gene Knockdown Techniques
- Hedgehog Proteins/metabolism
- Histone Acetyltransferases/genetics*
- Histone Acetyltransferases/metabolism*
- Nerve Tissue Proteins/genetics*
- Nerve Tissue Proteins/metabolism*
- RNA, Transfer/genetics
- Signal Transduction
- Thiouridine/analogs & derivatives
- Thiouridine/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 32023806 Full text @ Int. J. Mol. Sci.
Citation
Rojas-Benítez, D., L Allende, M. (2020) Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development. International Journal of Molecular Sciences. 21(3):.
Abstract
Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm5s2U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping