|ZFIN ID: ZDB-PUB-200207-22|
Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
Rojas-Benítez, D., L Allende, M.
|Source:||International Journal of Molecular Sciences 21(3): (Journal)|
|Keywords:||elongator complex, tRNA modification, zebrafish|
|PubMed:||32023806 Full text @ Int. J. Mol. Sci.|
Rojas-Benítez, D., L Allende, M. (2020) Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development. International Journal of Molecular Sciences. 21(3):.
ABSTRACTTransfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm5s2U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism.