ZFIN ID: ZDB-PUB-200207-22
Elongator Subunit 3 (Elp3) Is Required for Zebrafish Trunk Development
Rojas-Benítez, D., L Allende, M.
Date: 2020
Source: International Journal of Molecular Sciences   21(3): (Journal)
Registered Authors:
Keywords: elongator complex, tRNA modification, zebrafish
MeSH Terms:
  • Animals
  • Gene Knockdown Techniques
  • Hedgehog Proteins/metabolism
  • Histone Acetyltransferases/genetics*
  • Histone Acetyltransferases/metabolism*
  • Nerve Tissue Proteins/genetics*
  • Nerve Tissue Proteins/metabolism*
  • RNA, Transfer/genetics
  • Signal Transduction
  • Thiouridine/analogs & derivatives
  • Thiouridine/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism
PubMed: 32023806 Full text @ Int. J. Mol. Sci.
Transfer RNAs (tRNAs) are the most post-transcriptionally modified RNA species. Some of these modifications, especially the ones located in the anti-codon loop, are required for decoding capabilities of tRNAs. Such is the case for 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U), synthetized by the Elongator complex. Mutants for its sub-units display pleiotropic phenotypes. In this paper, we analyze the role of elp3 (Elongator catalytic sub-unit) in zebrafish development. We found that it is required for trunk development; elp3 knock-down animals presented diminished levels of mcm5s2U and sonic hedgehog (Shh) signaling activity. Activation of this pathway was sufficient to revert the phenotype caused by elp3 knockdown, indicating a functional relationship between Elongator and Shh through a yet unknown molecular mechanism.