ZFIN ID: ZDB-FIG-190723-468
Tobia et al., 2019 - Atypical Chemokine Receptor 3 Generates Guidance Cues for CXCL12-Mediated Endothelial Cell Migration. Frontiers in immunology   10:1092 Full text @ Front Immunol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Figure 3

Effect of ackr3b knockdown on arterial/venous differentiation and lateral dorsal aorta (LDA) bifurcation. (A–F) WISH analysis of the arterial marker ephrin-B2a(A,B) and venous markers ephb4a(C,D) and flt4(E,F) expression was performed at 28 hpf on zebrafish embryos injected with std-MO (A,C,E) or ackr3b-MO1 (B,D,F). Arterial and venous marker expression is normal in ackr3b morphants when compared to controls. (G,H) WISH analysis of cadherin 5 expression was performed at 30 hpf on zebrafish embryos injected with std-MO (G) or ackr3b-MO1 (H) to investigate LDA formation. Note the interruption of LDA in ackr3b morphants [asterisk in H]. The number of embryos presenting the showed phenotype in respect to the total number of analyzed embryos is shown in each panel. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 AB standard conditions Prim-15 blood vasculature ISH
AB + MO9-ackr3b standard conditions Prim-15 blood vasculature ISH
efnb2a AB standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
AB + MO9-ackr3b standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
ephb4a AB standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
AB + MO9-ackr3b standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
flt4 AB standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
AB + MO9-ackr3b standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO9-ackr3b standard conditions Prim-15 lateral dorsal aorta disconnected, abnormal
Prim-15 lateral dorsal aorta structurally discontinuous, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol