ZFIN Gene: ephb4a

ZFIN ID: ZDB-GENE-990415-62

ZFIN ID: ZDB-GENE-990415-62

Gene Name:

eph receptor B4a

Gene Symbol:

ephb4a Nomenclature History

Previous Names:

ephB4 (1), ephB4a, cb646 (1), rtk5

Gene Type:

protein_coding_gene

Location:

Chr: 5 Mapping Details/Browsers

Description:

Predicted to have transmembrane-ephrin receptor activity. Involved in convergent extension; ephrin receptor signaling pathway; and vasculature development. Predicted to localize to the integral component of plasma membrane; neuron projection; and receptor complex. Human ortholog(s) of this gene implicated in hereditary lymphedema. Is expressed in several structures, including axis; cardiovascular system; digestive system; nervous system; and neural tube. Orthologous to human EPHB4 (EPH receptor B4).

Genome Resources:

Alliance (1), Gene:30688 (1), Ensembl(GRCz11):ENSDARG00000100725 (1)

GENE EXPRESSION
All Expression Data:	37 figures from 30 publications
Directly Submitted Expression Data:	6 figures (39 images) from Thisse et al., 2001 [cb646]
Wild-type Stages, Structures:	Gastrula:50%-epiboly (5.25h-5.66h) to Adult (90d-730d, breeding adult)
	artery axial blood vessel axis basal plate midbrain region blood vasculature blood vessel endothelium cardiovascular system caudal vein plexus diencephalon dorsal aorta epiphysis female organism brain floor plate gut hindbrain hindbrain neural plate hypochord immature eye lateral line system lens margin midbrain midbrain neural keel neural tube notochord olfactory placode optic tectum otic vesicle pectoral fin bud pectoral fin musculature pharyngeal arch 3-7 skeleton pharynx posterior cardinal vein presumptive telencephalon rhombomere 2 rhombomere 5 rhombomere 6 spinal cord tail bud tegmentum telencephalon trunk vasculature artery unspecified vein ventral mesenchyme ventral mesoderm whole organism
Curated Microarray Expression:	GEO (1) , Expression Atlas (1)

MUTATIONS AND SEQUENCE TARGETING REAGENTS

Allele	Type	Localization	Consequence	Mutagen	Suppliers
hu3378	Unknown	Unknown	Unknown	ENU	European Zebrafish Resource Center (EZRC) (order this)
hu3445	Point Mutation	Unknown	Premature Stop	ENU	Zebrafish International Resource Center (ZIRC) (order this)
la015774Tg	Transgenic Insertion	Unknown	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
la025269Tg	Transgenic Insertion	Unknown	Unknown	DNA	Zebrafish International Resource Center (ZIRC) (order this)
sa11431	Point Mutation	Unknown	Premature Stop	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)
sa13275	Point Mutation	Unknown	Premature Stop	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)
sa30869	Point Mutation	Unknown	Premature Stop	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)
sa33770	Point Mutation	Unknown	Premature Stop	ENU	European Zebrafish Resource Center (EZRC) (order this) Zebrafish International Resource Center (ZIRC) (order this)

Sequence Targeting Reagents

Targeting Reagent	Created Alleles	Publications
MO1-ephb4a	N/A	2
MO2-ephb4a	N/A	2
MO3-ephb4a	N/A	2
MO4-ephb4a	N/A	1

PHENOTYPE

Data:	8 figures from 5 publications
Observed in:	blood vessel morphogenesis caudal vein plexus cranial vasculature dorsal aorta dorsal longitudinal vein eye intersegmental artery intersegmental vein intersegmental vessel mesencephalic vein post-vent region blood circulation post-vent region post-vent vasculature

DISEASE ASSOCIATED WITH ephb4a HUMAN ORTHOLOG

Disease Ontology Term	Multi-Species Data	OMIM Term	OMIM Phenotype ID
		Capillary malformation-arteriovenous malformation 2	618196
		Lymphatic malformation 7	617300

DISEASE ASSOCIATED WITH ephb4a VIA EXPERIMENTAL MODELS

Human Disease	Fish	Conditions	Citations
lymphatic system disease	y1Tg + MO4-ephb4a	standard conditions	Li et al., 2018
	y1Tg + MO2-ephb4a	standard conditions	Li et al., 2018

GENE ONTOLOGY

Ontology	GO Term
Biological Process	blood vessel development (more)
Cellular Component	integral component of membrane (more)
Molecular Function	ATP binding (more)

GO Terms (all 24)

PROTEIN FAMILIES, DOMAINS AND SITES
Type	InterPro ID	Name
Active_site	IPR008266	Tyrosine-protein kinase, active site
Binding_site	IPR017441	Protein kinase, ATP binding site
Conserved_site	IPR001426	Tyrosine-protein kinase, receptor class V, conserved site
Domain	IPR000719	Protein kinase domain
Domain	IPR001090	Ephrin receptor ligand binding domain
Domain	IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain
Domain	IPR001660	Sterile alpha motif domain
Domain	IPR003961	Fibronectin type III
Domain	IPR011641	Tyrosine-protein kinase ephrin type A/B receptor-like
Domain	IPR020635	Tyrosine-protein kinase, catalytic domain
Domain	IPR027936	Ephrin receptor, transmembrane domain
Domain	IPR034290	Ephrin type-B receptor 4, ligand binding domain
Domain	IPR037636	EPH-B4, SAM domain
Family	IPR016257	Ephrin receptor type-A /type-B
Homologous_superfamily	IPR008979	Galactose-binding-like domain superfamily
Homologous_superfamily	IPR009030	Growth factor receptor cysteine-rich domain superfamily
Homologous_superfamily	IPR011009	Protein kinase-like domain superfamily
Homologous_superfamily	IPR013761	Sterile alpha motif/pointed domain superfamily
Homologous_superfamily	IPR013783	Immunoglobulin-like fold
Homologous_superfamily	IPR036116	Fibronectin type III superfamily

TRANSCRIPTS
Type	Name	Length (nt)	Analysis
mRNA	ephb4a-201 (1)	2940	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
	ephb4a-202 (1)	1308	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT

Browsers: UCSC, NCBI, Ensembl, ZFIN

INTERACTIONS AND PATHWAYS

ANTIBODIES No data available

PLASMIDS No data available

CONSTRUCTS WITH SEQUENCES FROM ephb4a

Construct	Regulatory Regions	Coding Sequences	Species	Tg Lines	Publications
Tg(UAS:ephb4a_K662R,cryaa:Cerulean)	cryaa, UAS	Cerulean, ephb4a	Danio rerio	1	2

MARKER RELATIONSHIPS

ephb4a Contained in:	[Fosmid] CH1073-264G23 (1), ZFOS-1566H6 (1)
ephb4a Contains:	[STS] unp162
ephb4a Encodes:	[EST] cb646 (1) (order this) [cDNA] MGC:136748 (1), MGC:171234 (1)

SEQUENCE INFORMATION

Type	Accession #	Length (nt/aa)	Analysis
RNA	RefSeq:NM_131414 (1)	3055 nt	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Genomic	GenBank:FO904976 (1)	34139 nt	Select Tool MegaBLAST Ensembl
Polypeptide	UniProtKB:O73875 (1)	987 aa	Select Tool ZFIN BLAST SIB BLAST Ensembl Protein Blast

Sequence Information (all 36)

ORTHOLOGY for ephb4a ( Chr: 5 )

CITATIONS (94)

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