ZFIN ID: ZDB-FIG-190723-467
Tobia et al., 2019 - Atypical Chemokine Receptor 3 Generates Guidance Cues for CXCL12-Mediated Endothelial Cell Migration. Frontiers in immunology   10:1092 Full text @ Front Immunol
Anatomical Terms:
Stage Range: 50%-epiboly to Prim-5

Figure 2

WISH analysis of ackr3b and cxcr4a expression during zebrafish embryo development. WISH analysis of ackr3b(A–H) and cxcr4a(I–J) mRNA expression was performed at the indicated stages of development. (A–C,E,G,I,J) Lateral view of whole-mount embryos. (D) Coronal cross section through the trunk highlighted as a dashed vertical bar in (C), anterior to the top; arrows indicate somitic ackr3b expression. (F,H) Transverse cross sections through the trunk highlighted as dashed bars in (E,G), respectively. Arrows in (F,H) indicate ackr3b-positive developing gut and the basal part of the somites, respectively. (A–C,E,G,I,J) Scale bar: 100 μm. (D,F,H) Scale bar: 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ackr3a AB standard conditions 50%-epiboly presumptive endoderm ISH
75%-epiboly endoderm ISH
14-19 somites rhombomere 3 ISH
14-19 somites rhombomere 5 ISH
14-19 somites rhombomere 6 ISH
14-19 somites somite ISH
20-25 somites rhombomere 3 ISH
20-25 somites rhombomere 5 ISH
20-25 somites rhombomere 6 ISH
20-25 somites somite ISH
Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 5 ISH
Prim-5 rhombomere 6 ISH
Prim-5 somite ISH
Prim-5 spinal cord ISH
cxcr4a AB standard conditions 20-25 somites blood vessel endothelial cell ISH
Prim-5 dorsal aorta blood vessel endothelial cell ISH
Prim-5 intersegmental vessel blood vessel endothelial cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Frontiers in immunology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Immunol