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ZFIN ID: ZDB-FIG-190410-8
Meng et al., 2017 - The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors. Nature communications   8:14640 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Pec-fin

Fig. 3 Lack of stat4 represses emergence of PAA angioblasts.

(a,b) tie1 transcripts expressing PAAs are visualized in the control and stat4 mutants at 60 hpf. (c,d) Resin sections (10 μm per section) of wild-type and stat4 mutant embryos with tie1 in situ hybridization at 60 hpf. (e–h) Analysis of tie1 expression in pharyngeal angioblastic cords is conducted in the control embryos (e), stat4 mutants (f), stat4 morphant embryos (g) and embryos with stat4 mRNA and stat4 morpholino injection (h) at 44 hpf by in situ hybridization. (k) Proportional quantification of embryos with defective PAA angioblast cords in tie1 staining assays, Kruskal–Wallis test with the Dunn’s multiple comparison test, **P<0.01, n.s.: P>0.05. (i,j,m,n) The etv2 and scl transcripts are evaluated by in situ hybridization in the control and stat4 mutants at 38 hpf. (o) Percentage of embryos with defective PAA angioblastic cords in etv2 and scl staining assays, error bars indicate the s.d., Kruskal–Wallis test, **P=0.0058, n=30 per each group. (l,p) tcf21+ PAAs are visualized in the control and stat4 mutants at 28 hpf. Scale bars, 50 μm. a–d, n=23 embryos in each group; e–h, n=32 embryos in each group; l,p, n=26 embryos per group.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etsrp AB/TU standard conditions Prim-25 aortic arch embryonic blood vessel endothelial progenitor cell ISH
stat4zf2070/zf2070 standard conditions Prim-25 aortic arch embryonic blood vessel endothelial progenitor cell ISH
tal1 AB/TU standard conditions Prim-25 aortic arch embryonic blood vessel endothelial progenitor cell ISH
stat4zf2070/zf2070 standard conditions Prim-25 aortic arch embryonic blood vessel endothelial progenitor cell ISH
tcf21 AB/TU standard conditions Prim-5 aortic arch myoblast ISH
stat4zf2070/zf2070 standard conditions Prim-5 aortic arch myoblast ISH
tie1 AB/TU standard conditions High-pec aortic arch angioblastic mesenchymal cell ISH
Pec-fin aortic arch 3 angioblastic mesenchymal cell ISH
Pec-fin aortic arch 4 angioblastic mesenchymal cell ISH
Pec-fin aortic arch 5 angioblastic mesenchymal cell ISH
Pec-fin aortic arch 6 angioblastic mesenchymal cell ISH
AB/TU + MO1-stat4 standard conditions High-pec aortic arch angioblastic mesenchymal cell ISH
stat4zf2070/zf2070 standard conditions High-pec aortic arch angioblastic mesenchymal cell ISH
Pec-fin aortic arch 3 angioblastic mesenchymal cell ISH
Pec-fin aortic arch 4 angioblastic mesenchymal cell ISH
Pec-fin aortic arch 5 angioblastic mesenchymal cell ISH
Pec-fin aortic arch 6 angioblastic mesenchymal cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB/TU + MO1-stat4 standard conditions High-pec aortic arch 3 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 3 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch 4 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 4 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch 5 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 5 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch 6 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 6 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch angioblastic mesenchymal cell tie1 expression decreased amount, abnormal
stat4zf2070/zf2070 standard conditions Prim-25 aortic arch embryonic blood vessel endothelial progenitor cell etsrp expression decreased amount, abnormal
Prim-25 aortic arch embryonic blood vessel endothelial progenitor cell tal1 expression decreased amount, abnormal
High-pec aortic arch 3 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 3 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch 4 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 4 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch 5 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 5 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch 6 angioblast cell differentiation decreased occurrence, abnormal
High-pec aortic arch 6 vasculogenesis decreased occurrence, abnormal
High-pec aortic arch angioblastic mesenchymal cell tie1 expression decreased amount, abnormal
Pec-fin aortic arch 3 angioblast cell differentiation decreased occurrence, abnormal
Pec-fin aortic arch 3 angioblastic mesenchymal cell tie1 expression decreased amount, abnormal
Pec-fin aortic arch 4 angioblast cell differentiation decreased occurrence, abnormal
Pec-fin aortic arch 4 angioblastic mesenchymal cell tie1 expression decreased amount, abnormal
Pec-fin aortic arch 5 angioblast cell differentiation decreased occurrence, abnormal
Pec-fin aortic arch 5 angioblastic mesenchymal cell tie1 expression decreased amount, abnormal
Pec-fin aortic arch 6 angioblast cell differentiation decreased occurrence, abnormal
Pec-fin aortic arch 6 angioblastic mesenchymal cell tie1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.