|ZFIN ID: ZDB-IMAGE-180816-5|
Etv2 depletion by caged MO photoactivation results in reduction of prox1a:GFP+ lymphatic progenitor cells. TgBAC(prox1a:KalT4-UAS:uncTagRFP); Tg(UAS:GFP) live embryos were imaged by confocal microscopy at 32 hpf. The number of prox1a:GFP+ cells is significantly reduced, 1.8 ± 0.32 cells, in embryos where etv2 is depleted at 24 hpf by caged MO photoactivation (n = 12) (B), compared to uninjected embryos (A) (5.1 ± 0.38 cells, n = 13, p < 0.0001, two tailed student's t-test). Arrows indicate prox1a:GFP+ endothelial cells. (C) Quantification of prox1a:GFP positive cells across the width of 9 somites in the trunk region. Mean±SEM is shown. Individual data points are shown in Fig. S4B. This experiment has been replicated twice.
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