ZFIN ID: ZDB-FIG-180515-3
Gagnon et al., 2017 - The primary role of zebrafish nanog is in extra-embryonic tissue.. Development (Cambridge, England)   145(1) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Sphere
PHENOTYPE:
Fish:
Observed In:
Stage: Sphere

Fig. 3

Defects in early zygotic gene expression in MZnanog embryos. (A) In situ hybridization for bmp2b and vox expression in wild-type and MZnanog embryos at sphere (4 hpf) stage. (B) Fold expression change for the indicated genes comparing wild type and MZnanog at sphere stage using RT-qPCR. Error bars show s.d. for three technical replicates (ten embryos per replicate). (C) Differential expression of early zygotic genes, comparing wild type and MZnanog at sphere stage using RNAseq. All genes are in gray, early zygotic genes (MZT genes) in black were previously defined (Lee et al., 2013). (D) Differential expression of maternally provided genes, comparing wild type and MZnanog at sphere stage using RNAseq. All genes are in gray, maternally provided genes in black were previously defined (Rabani et al., 2014). Only those genes with wild-type mRNA expression >1 FPKM are plotted. Genes are categorized as up- or downregulated if their expression in MZnanog differs more than 2-fold from that in wild type. (E) Distribution of fold changes between wild-type and MZnanog embryos for all early zygotic (blue) and maternal (red) genes visualized using a kernel density estimation. The displayed P-value comparing these two sets was calculated using a Student's two-tailed t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
blf nanoga166/a166 standard conditions Sphere whole organism RTPCR
bmp2b WT standard conditions Sphere whole organism ISH
nanoga166/a166 standard conditions Sphere whole organism ISH
Sphere whole organism RTPCR
foxa3 nanoga166/a166 standard conditions Sphere whole organism RTPCR
tbx16 nanoga166/a166 standard conditions Sphere whole organism RTPCR
vent nanoga166/a166 standard conditions Sphere whole organism RTPCR
vox WT standard conditions Sphere whole organism ISH
nanoga166/a166 standard conditions Sphere whole organism RTPCR
Sphere whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nanoga166/a166 standard conditions Sphere whole organism foxa3 expression decreased amount, abnormal
Sphere whole organism vox expression decreased amount, abnormal
Sphere whole organism bmp2b expression decreased amount, abnormal
Sphere whole organism vox expression decreased amount, abnormal
Sphere whole organism vent expression decreased amount, abnormal
Sphere whole organism bmp2b expression decreased amount, abnormal
Sphere whole organism tbx16 expression decreased amount, abnormal
Sphere whole organism blf expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development