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ZFIN ID: ZDB-FIG-180320-16
Kim et al., 2017 - Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8. Scientific Reports   7:13278 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Bud to 10-13 somites

Fig. 4

Loss of otg function affects AP-DV patterning and nuclear β-catenin localization. (A–C) Doral views, anterior to the left. Expression patterns of an anterior neural marker six3 and a posterior neural marker hoxa1 in control (A, 12/12, 100%), OTG mRNA-injected (B, 17/20, 85%) or otg MO-injected (C, 16/18, 89%) embryo at tail bud stage (10 hpf). (D–F) Doral views, posterior to the right. Expression pattern of a posterior mesoderm marker spt in control (D, 10/10, 100%), OTG mRNA-injected (E, 18/20, 90%) and otg MO-injected (F, 20/23, 87%) embryo at 10 somite stage. (G–J) Views of animal pole cells at blastula stage. Immunostaining for β-catenin and fluorescence intensity profile along the white lines in control (G), wnt8 mRNA-injected (H), wnt8 and OTG mRNA-injected (I), or wnt8 mRNA and otg MO-injected (J) embryo.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hoxb1b WT control Bud neuroectoderm posterior region ISH
WT + MO1-prr7 control Bud neuroectoderm posterior region ISH
six3b WT control Bud neuroectoderm anterior region ISH
WT + MO1-prr7 control Bud neuroectoderm anterior region ISH
tbx16 WT control 10-13 somites segmental plate ISH
WT + MO1-prr7 control 10-13 somites segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prr7 control Bud neuroectoderm anterior region six3b expression decreased distribution, abnormal
Bud neuroectoderm posterior region hoxb1b expression increased distribution, abnormal
10-13 somites segmental plate tbx16 expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.