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ZFIN ID: ZDB-PUB-171019-21
Ottogi Inhibits Wnt/β-catenin Signaling by Regulating Cell Membrane Trafficking of Frizzled8
Kim, H.T., Lee, M.S., Jeong, Y.M., Ro, H., Kim, D.I., Shin, Y.H., Kim, J.E., Hwang, K.S., Choi, J.H., Bahn, M., Lee, J.J., Lee, S.H., Bae, Y.K., Lee, J.S., Choi, J.K., Kim, N.S., Yeo, C.Y., Kim, C.H.
Date: 2017
Source: Scientific Reports   7: 13278 (Journal)
Registered Authors: Bae, Young Ki, Choi, Jung-Hwa, Kim, Cheol-Hee, Kim, Dong-Il, Kim, Hyun-Taek, Lee, Mi-Sun
Keywords: none
MeSH Terms:
  • Animals
  • Cell Membrane/metabolism*
  • DNA, Complementary/genetics
  • Embryonic Development/genetics
  • Endoplasmic Reticulum/metabolism
  • Gene Expression
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Glycosylation
  • Humans
  • Phenotype
  • Protein Binding
  • Protein Transport
  • Receptors, Cell Surface/metabolism*
  • Transcriptome
  • Wnt Signaling Pathway*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 29038508 Full text @ Sci. Rep.
Wnt signaling controls critical developmental processes including tissue/body patterning. Here we report the identification of a novel regulator of Wnt signaling, OTTOGI (OTG), isolated from a large-scale expression screening of human cDNAs in zebrafish embryos. Overexpression of OTG in zebrafish embryos caused dorso-anteriorized phenotype, inhibited the expression of Wnt target genes, and prevented nuclear accumulation of β-catenin. Conversely, knockdown of zebrafish otg using specific antisense morpholino promoted nuclear accumulation of β-catenin and caused ventralization. However, OTG failed to rescue headless-like phenotype induced by inhibition of GSK-3β activity, suggesting that OTG acts upstream of GSK-3β. OTG bound specifically to Frizzled8 (Fz8) receptor and caused retention of Fz8 in the endoplasmic reticulum possibly by preventing N-linked glycosylation of Fz8. Taken together, our data indicate that OTG functions as a novel negative regulator of Wnt signaling during development by the modulation of cell surface expression of Fz receptor.