FIGURE

Fig. S3

ID

ZDB-FIG-171013-11

Publication

Sassen et al., 2017 - Embryonic zebrafish primary cell culture for transfection and live cellular and subcellular imaging

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Fig. S3

Activation of molecular marker expression after plating. (A) Double transgenic Tg(ptf1a:eGFP) / Tg(ca8:FyntagRFP)bz4Tg embryos show only GFP expression at 2 dpf. Scale bar, 100 μm. (B) No RFP expression could be detected in a primary cell culture obtained from double transgenic Tg(ptf1a:eGFP) / Tg(ca8:FyntagRFP)bz4Tg embryos 60 min after plating. Scale bar, 100 μm. (C) and (D) At 1 dap, few cells were observed in which the expression of the transgene ca8:FyntagRFP was activated. Scale bar, 10 μm. Shown is a collection of the images of two preparations with comparable results.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 430(1), Sassen, W.A., Lehne, F., Russo, G., Wargenau, S., Dübel, S., Köster, R.W., Embryonic zebrafish primary cell culture for transfection and live cellular and subcellular imaging, 18-31, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.