ZFIN ID: ZDB-FIG-170921-56
Gallagher et al., 2017 - Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Developmental Biology   429(1):225-239 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 6

pnrc2 and the nonsense-mediated decay effector Upf1 promote decay of cyclic mRNA. Injection of low dose pnrc2 sbMO (2 ng) has little to no effect on her1 expression (A, C), contrasting with the expected her1 misexpression observed after injection of moderate dose pnrc2 sbMO (6 ng) (B). Low dose injection of upf1 sbMO (0.25 ng) also has little effect on her1 expression (D), but when combined with a low dose of pnrc2 sbMO (2 ng), her1 misexpression is observed in about 50% of injected embryos (E). The proportion affected in each condition is plotted on a bar graph that indicates significant differences between single morphants, double morphants, and controls (F). sbMO = splice-blocking MO; **** = p<0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her1 WT standard conditions 14-19 somites segmental plate ISH
WT + MO2-pnrc2 standard conditions 14-19 somites segmental plate ISH
WT + MO2-pnrc2 + MO2-upf1 standard conditions 14-19 somites segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-pnrc2 standard conditions 14-19 somites segmental plate her1 expression increased amount, abnormal
WT + MO2-pnrc2 + MO2-upf1 standard conditions 14-19 somites segmental plate her1 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.