ZFIN ID: ZDB-FIG-170921-51
Gallagher et al., 2017 - Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Developmental Biology   429(1):225-239 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 14-19 somites

Fig. 1

The tortugab644 allele is a 1.46 Mb deficiency that includes the pnrc2 gene. Haploid-based mapping revealed that the tortuga lesion lies 0.30 cM to the left (4/1434 recombinants) and 0.37 cM to the right (4/1083 recombinants) of the SSLP markers z13511 and z9511, respectively (A). The extent of the deletion was refined by PCR-based screening of regions within and around the deletion interval from pooled genomic DNA samples of 10 wild-type (WT) and 10 tortuga mutant embryos (B); regions that fail to amplify in mutants are deleted in the torb644 allele (A, B). Among BACs spanning the deletion interval, only BAC AL844887 restores her1 expression when injected into tor mutants (A; Fig S1A-D). In wild-type embryos, her1 is expressed in a striped pattern (n=54/54) (C). In contrast, embryos injected with 6 ng of pnrc2 splice-blocking morpholino (sbMO) have a tortuga-like her1 expression defect (n=37/39) (D). When pnrc2 mRNA is co-injected with 4 ng pnrc2 sbMO, her1 expression is partially restored in a dose-dependent manner (n=3/19 WT her1 expression, 150 pg pnrc2 mRNA; n=7/17 WT her1 expression, 600 pg pnrc2 mRNA) (E; Table 1). Alignment of vertebrate Pnrc2 amino acid sequences reveals a conserved 10 amino acid N-terminus (purple) and conserved C-terminal SRC-Homology 3 (SH3) (red) and Nuclear Receptor (NR box) domains (green) (F). Clustal alignments were performed in consultation with published alignments for vertebrate Pnrc2 (Valkov et al., 2016).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her1 WT standard conditions 14-19 somites segmental plate ISH
WT + MO2-pnrc2 standard conditions 14-19 somites segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-pnrc2 standard conditions 14-19 somites segmental plate her1 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.