ZFIN ID: ZDB-FIG-170921-55
Gallagher et al., 2017 - Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation. Developmental Biology   429(1):225-239 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: 14-19 somites

Fig. 5

Pnrc2-mediated decay functions via 3’UTR recognition and does not require Dicer-dependent miRNAs. Stably transgenic embryos carrying the hsp70l:Venus her1 3’UTR reporter were injected at the 1-cell stage with pnrc2 sbMO or reserved as uninjected controls. Embryos were then raised to mid-segmentation stage, heat-shocked for 15 min, then collected at the indicated minutes post-heat-shock (pHS) and processed by Venus in situ hybridization (n=65, 6 ng pnrc2 sbMO; n=55, uninjected controls) (A-F). Venus transcripts are not detected in the absence of heat-shock (n=10) (not shown). To determine whether Dicer-generated miRNAs contribute to her1 mRNA decay, MZdicer mutants (n=11) and wild-type controls (n=10) were raised to mid-segmentation stages (16–18 hpf) and processed by her1 in situ hybridization (G, H). At this timepoint, MZdicer mutants are developmentally delayed relative to wild-type controls (Giraldez et al., 2005), and thus have a different overall shape.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her1 WT standard conditions 14-19 somites segmental plate ISH
dicer1hu715/hu715 standard conditions 14-19 somites segmental plate ISH
Venus oz40Tg(AB) heat shock 14-19 somites whole organism ISH
oz40Tg + MO2-pnrc2(AB) heat shock 14-19 somites whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
oz40Tg(AB) heat shock 14-19 somites whole organism Venus expression decreased amount, abnormal
oz40Tg + MO2-pnrc2(AB) heat shock 14-19 somites whole organism Venus expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 429(1), Gallagher, T.L., Tietz, K.T., Morrow, Z.T., McCammon, J.M., Goldrich, M.L., Derr, N.L., Amacher, S.L., Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation, 225-239, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.