ZFIN ID: ZDB-FIG-170912-4
Xie et al., 2016 - S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence. Developmental Biology   414:228-43 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 1

S1pr2/Gα13 signaling is required for the migration, but not differentiation, of endocardial precursors. (A-D) Projections of four single XY confocal Z-planes of 26 hpf-Tg(myl7: memGFP/kdrl: mCherry) embryos raised at 28.5 °C, showing the myocardium (green) and endothelial cells (magenta). Arrows indicate the presumptive endocardium among the endothelial cells, which formed inside of cardiac population in control embryos (A), but outside of cardiac population in the mutant (B, C). (E) Frequencies of heart defects in 26-hpf embryos injected with gna13a/b MO, s1pr2 MO, or s1pr2 MO and a human Gα13 RNA (GNA13) raised at 28.5 °C, as assessed by epifluorescence imaging. *, P<0.01. Bars indicate the mean±s.e.m. (F-K) Expression of nfatc1 and cdh5, as detected by whole-mount in situ hybridization (ISH), in control, mil mutant, and gna13a/b MO-injected embryos. White arrowheads: endocardial populations. All images are dorsoanterior views with anterior up. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 WT control Prim-5 vascular endothelium ISH
WT + MO1-gna13a + MO1-gna13b standard conditions Prim-5 vascular endothelium ISH
s1pr2m93/m93 standard conditions Prim-5 vascular endothelium ISH
GFP ui6Tg ; is5Tg control Prim-5 myocardium IFL
ui6Tg ; is5Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-5 myocardium IFL
ui6Tg ; is5Tg + MO1-s1pr2 standard conditions Prim-5 myocardium IFL
mCherry ui6Tg ; is5Tg control Prim-5 endocardium IFL
Prim-5 endothelial cell IFL
ui6Tg ; is5Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-5 endocardium IFL
Prim-5 endothelial cell IFL
ui6Tg ; is5Tg + MO1-s1pr2 standard conditions Prim-5 endocardium IFL
Prim-5 endothelial cell IFL
nfatc1 WT control Prim-5 endocardium ISH
WT + MO1-gna13a + MO1-gna13b standard conditions Prim-5 endocardium ISH
s1pr2m93/m93 standard conditions Prim-5 endocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gna13a + MO1-gna13b standard conditions Prim-5 endocardium unfused from endocardium, abnormal
s1pr2m93/m93 standard conditions Prim-5 endocardium unfused from endocardium, abnormal
ui6Tg ; is5Tg + MO1-gna13a + MO1-gna13b standard conditions Prim-5 endocardium mislocalised, abnormal
ui6Tg ; is5Tg + MO1-s1pr2 standard conditions Prim-5 endocardium mislocalised, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 414, Xie, H., Ye, D., Sepich, D., Lin, F., S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence, 228-43, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.