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ZFIN ID: ZDB-FIG-170912-11
Xie et al., 2016 - S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence. Developmental Biology   414:228-43 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to 26+ somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to 26+ somites

Fig. 8

Defects in myocardial migration in the context of global Gα13 deficiency are partially rescued when endocardial precursors are eliminated. (A-F′) Snapshots of movies taken of Tg(fli: EGFP/nkx2.5: Kaede) embryos injected with gna13a/b MO, either alone (A-F, n=8) or with the etv2 MO (A′-F′, n=8) (Supplementary movie 5), showing the migration of endocardial (green, outlined by white dots, arrowheads) and myocardial (magenta, outlined by yellow dots and arrowheads) precursors at the stages indicated. 10 s-embryos were exposed to UV light for 1 min to convert Kaede fluorescence from green to red (presented as magenta). Asterisks: Non-myocardial cells labeled with Nkx2.5-Kaede; green arrowheads: non-endothelial cell population labeled with Fli-EGFP, which was also reported previously. Dorsoanterior views (Craig et al., 2015). (G-J) Expression of myl7 and cdh5 in 26 s embryos, as detected by ISH. Yellow arrowheads: myocardial cells; white arrowheads: endothelial cells. All images are dorsoanterior views with anterior up. (K) Distance between the two populations of endocardial cells in embryos injected with gna13a/b MO, either alone (A-F) or with the etv2 MO (A′-F′), at indicated stages. Data are mean±s.e.m. #: P<0.05 between the two experimental groups. (L) Frequencies of heart defects (two separated and fused hearts) in the indicated embryos in G-J. The number of embryos in each sample is shown. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdh5 WT control 26+ somites endothelial cell ISH
WT + MO1-etv2 + MO1-gna13a + MO1-gna13b + MO2-etv2 standard conditions 26+ somites Not Detected endothelial cell ISH
WT + MO1-etv2 + MO2-etv2 standard conditions 26+ somites Not Detected endothelial cell ISH
WT + MO1-gna13a + MO1-gna13b standard conditions 26+ somites endothelial cell ISH
EGFP fb9Tg ; y1Tg + MO1-etv2 + MO1-gna13a + MO1-gna13b + MO2-etv2 standard conditions 10-13 somites to 20-25 somites Not Detected endothelial cell IFL
fb9Tg ; y1Tg + MO1-gna13a + MO1-gna13b standard conditions 10-13 somites to 20-25 somites endocardial precursor IFL
10-13 somites to 20-25 somites endothelial cell IFL
Kaede fb9Tg ; y1Tg + MO1-etv2 + MO1-gna13a + MO1-gna13b + MO2-etv2 standard conditions 10-13 somites to 20-25 somites myocardial precursor IFL
fb9Tg ; y1Tg + MO1-gna13a + MO1-gna13b standard conditions 10-13 somites to 20-25 somites myocardial precursor IFL
myl7 WT control 26+ somites myocardium ISH
WT + MO1-etv2 + MO1-gna13a + MO1-gna13b + MO2-etv2 standard conditions 26+ somites myocardium ISH
WT + MO1-etv2 + MO2-etv2 standard conditions 26+ somites myocardium ISH
WT + MO1-gna13a + MO1-gna13b standard conditions 26+ somites myocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-etv2 + MO1-gna13a + MO1-gna13b + MO2-etv2 standard conditions 26+ somites endothelial cell cdh5 expression absent, abnormal
26+ somites endothelial cell absent, abnormal
26+ somites myocardial precursor cardioblast migration to the midline involved in heart field formation occurrence, ameliorated
26+ somites myocardium fused with myocardium, ameliorated
WT + MO1-etv2 + MO2-etv2 standard conditions 26+ somites endothelial cell absent, abnormal
26+ somites endothelial cell cdh5 expression absent, abnormal
WT + MO1-gna13a + MO1-gna13b standard conditions 26+ somites myocardium unfused from myocardium, abnormal
fb9Tg ; y1Tg + MO1-etv2 + MO1-gna13a + MO1-gna13b + MO2-etv2 standard conditions 10-13 somites - 20-25 somites endothelial cell absent, abnormal
10-13 somites - 20-25 somites endothelial cell EGFP expression absent, abnormal
10-13 somites - 20-25 somites myocardial precursor cardioblast migration to the midline involved in heart field formation occurrence, ameliorated
fb9Tg ; y1Tg + MO1-gna13a + MO1-gna13b standard conditions 10-13 somites - 20-25 somites endocardial precursor cardioblast migration to the midline involved in heart field formation decreased occurrence, abnormal
10-13 somites - 20-25 somites endocardial progenitor cell migration to the midline involved in heart field formation decreased occurrence, abnormal
10-13 somites - 20-25 somites myocardial precursor cardioblast migration to the midline involved in heart field formation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 414, Xie, H., Ye, D., Sepich, D., Lin, F., S1pr2/Gα13 signaling regulates the migration of endocardial precursors by controlling endoderm convergence, 228-43, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.