ZFIN ID: ZDB-FIG-170314-10
Williams et al., 2017 - Cyp1b1 Regulates Ocular Fissure Closure Through a Retinoic Acid-Independent Pathway. Investigative ophthalmology & visual science   58:1084-1097 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 7

Cyp1b1 differentially regulated vsx2, shh, and pax6. Whole-mount in situ hybridization at 24 hpf demonstrated that vsx2 expression in the eye decreased in response to cyp1b1 overexpression (D, D') compared with Cyp1b1 MO knockdown (C, C'), control-injected (B, B'), and uninjected (A, A'') embryos. Note the appearance of tissue bridging the two optic cups in the cyp1b1 mRNA-injected embryo, as demarcated by vsx2 (arrow). Shha at 24 hpf showed increased expression in the midline floor plate between the eyes in embryos injected with cyp1b1 mRNA (arrowheads, H, H') compared with Cyp1b1 MO knockdown (G, G'), control -injected (F, F'), and uninjected (E, D') embryos. Pax6a and pax6b expression in the retina at 48 hpf decreased in response to cypb1 overexpression (L, L') compared with Cyp1b1 knockdown (K, K'), control-injected (J, J'), and uninjected (I, I') embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax6a WT control Long-pec retina ISH
WT standard conditions Long-pec retina ISH
WT + MO3-cyp1b1 standard conditions Long-pec retina ISH
pax6b WT control Long-pec retina ISH
WT standard conditions Long-pec retina ISH
WT + MO3-cyp1b1 standard conditions Long-pec retina ISH
shha WT control Prim-5 floor plate ISH
WT standard conditions Prim-5 floor plate ISH
WT + MO3-cyp1b1 standard conditions Prim-5 floor plate ISH
vsx2 WT control Prim-5 eye ISH
WT standard conditions Prim-5 eye ISH
WT + MO3-cyp1b1 standard conditions Prim-5 eye ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.