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ZIRC
ZFIN ID: ZDB-FIG-170314-9
Williams et al., 2017 - Cyp1b1 Regulates Ocular Fissure Closure Through a Retinoic Acid-Independent Pathway. Investigative ophthalmology & visual science   58:1084-1097 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6

Tyr expression is not influenced by alterations in cyp1b1 expression. In situ hybridization demonstrated that at 24 hpf, tyr was expressed in the migrating neural crest and retinal pigment epithelium in uninjected (A) and control-injected (B) embryos. At 48 hpf, tyr expression (A', A'', B', B'') followed the skin pigmentation pattern and was also observed in the cranial neural crest (arrowheads), retinal pigment epithelium, migrating neural crest cells in the ocular fissure (closed arrows), and iris stroma (open arrows). Knockdown of cyp1b1 did not alter tyr expression at 24 hpf, but did decrease tyr expression in the cranial neural crest (arrowhead) and ocular fissure (C, C') at 48 hpf. Overexpression of cyp1b1 did not alter tyr expression (D, D', D'') at 24 and 48 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tyr WT control Prim-5 migratory neural crest ISH
Prim-5 neural crest ISH
Prim-5 presumptive retinal pigmented epithelium ISH
Long-pec iris stroma ISH
Long-pec retinal pigmented epithelium ISH
WT standard conditions Prim-5 migratory neural crest ISH
Prim-5 neural crest ISH
Prim-5 presumptive retinal pigmented epithelium ISH
Long-pec iris stroma ISH
Long-pec retinal pigmented epithelium ISH
WT + MO3-cyp1b1 standard conditions Prim-5 migratory neural crest ISH
Prim-5 neural crest ISH
Prim-5 presumptive retinal pigmented epithelium ISH
Long-pec eye ISH
Long-pec migratory neural crest ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-cyp1b1 standard conditions Long-pec eye tyr expression increased amount, abnormal
Long-pec migratory neural crest tyr expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.