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ZIRC
ZFIN ID: ZDB-FIG-170309-12
Wircer et al., 2017 - Homeodomain protein Otp affects developmental neuropeptide switching in oxytocin neurons associated with a long-term effect on social behavior. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 2

otpa mutant reveals a new cluster of hypothalamic OXT neurons.

(AF) In situ hybridization of oxt mRNA in 5 day-old wild type (otpa+/+) and otpa−/− mutant on the background of a transgenic OXT reporter Tg(oxt:egfp) followed by confocal imaging (dorsal view, anterior to the top). otpa+/+;Tg(oxt:egfp) fish do not express detectable levels of oxt mRNA (A), but express EGFP in the posterior tuberculum (PT; B). otpa−/−; Tg(oxt:egfp) mutants express oxt mRNA (D) in oxt:egfp-positive cells of the PT (E). PT OXT neurons are indicated by arrowheads. Scale bar, 20 µm. (GI) A representative image (single confocal plane) of a transgenic [otpa+/+;Tg(oxt:egfp)] larvae, which was subjected to fluorescent in situ hybridization (FISH) with a single-molecule oxt mRNA probe (Stellaris) showing co-localization (arrowhead) of oxt in EGFP-labelled PT cells. NPO, neurosecretory preoptic area. Scale bar, 20 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP wz01Tg standard conditions Day 5 caudal tuberculum IHC
Day 5 preoptic area neurosecretory neuron IHC
wz01Tg ; otpam866/m866 standard conditions Day 5 caudal tuberculum IHC
Day 5 preoptic area neurosecretory neuron IHC
oxt wz01Tg standard conditions Day 5 caudal tuberculum ISH
Day 5 preoptic area neurosecretory neuron ISH
wz01Tg ; otpam866/m866 standard conditions Day 5 caudal tuberculum ISH
Day 5 preoptic area neurosecretory neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
wz01Tg ; otpam866/m866 standard conditions Day 5 caudal tuberculum oxt expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife