ZFIN ID: ZDB-FIG-170309-12 |
Genes: | |
---|---|
Fish: | |
Anatomical Terms: | |
Stage: | Day 5 |
Fish: | |
---|---|
Observed In: | |
Stage: | Day 5 |
Fig. 2 otpa mutant reveals a new cluster of hypothalamic OXT neurons. (A–F) In situ hybridization of oxt mRNA in 5 day-old wild type (otpa+/+) and otpa−/− mutant on the background of a transgenic OXT reporter Tg(oxt:egfp) followed by confocal imaging (dorsal view, anterior to the top). otpa+/+;Tg(oxt:egfp) fish do not express detectable levels of oxt mRNA (A), but express EGFP in the posterior tuberculum (PT; B). otpa−/−; Tg(oxt:egfp) mutants express oxt mRNA (D) in oxt:egfp-positive cells of the PT (E). PT OXT neurons are indicated by arrowheads. Scale bar, 20 µm. (G–I) A representative image (single confocal plane) of a transgenic [otpa+/+;Tg(oxt:egfp)] larvae, which was subjected to fluorescent in situ hybridization (FISH) with a single-molecule oxt mRNA probe (Stellaris) showing co-localization (arrowhead) of oxt in EGFP-labelled PT cells. NPO, neurosecretory preoptic area. Scale bar, 20 µm. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
---|---|---|---|---|---|---|
EGFP | wz01Tg | standard conditions | Day 5 | caudal tuberculum | IHC | |
Day 5 | preoptic area neurosecretory neuron | IHC | ||||
wz01Tg ; otpam866/m866 | standard conditions | Day 5 | caudal tuberculum | IHC | ||
Day 5 | preoptic area neurosecretory neuron | IHC | ||||
oxt | wz01Tg | standard conditions | Day 5 | caudal tuberculum | ISH | |
Day 5 | preoptic area neurosecretory neuron | ISH | ||||
wz01Tg ; otpam866/m866 | standard conditions | Day 5 | caudal tuberculum | ISH | ||
Day 5 | preoptic area neurosecretory neuron | ISH |
Fish | Conditions | Stage | Phenotype |
---|---|---|---|
wz01Tg ; otpam866/m866 | standard conditions | Day 5 | caudal tuberculum oxt expression increased amount, abnormal |
Acknowledgments: | ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife |
---|