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ZIRC
ZFIN ID: ZDB-FIG-170309-16
Wircer et al., 2017 - Homeodomain protein Otp affects developmental neuropeptide switching in oxytocin neurons associated with a long-term effect on social behavior. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult

Fig. 3

Posterior tuberculum (PT) OXT neurons represent a new cluster of zebrafish parvocellular neurons.

Immunostaining and confocal imaging of either 5 day-old larva (A) or adult (B; 150 ┬Ám sagittal section) transgenic OXT reporter Tg(oxt:egfp) with a tyrosine hydroxylase (TH) antibody which serves as an anatomical landmark. The soma size of OXT neurons was measured using FIJI image-processing package in three larvae (C) and two adults (D) and plotted as a function of their relative anterior-posterior position. Each individual animal is marked by a different shape. NPO and PT neurons are labeled in green and orange, respectively. NPO, neurosecretory preoptic area; PM, magnocellular preoptic nucleus; PMg, gigantocellular part of magnocellular preoptic nucleus; PPa, parvocellular preoptic nucleus - anterior part; PT, posterior tuberculum; TH, tyrosine hydroxylase.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP wz01Tg standard conditions Day 5 caudal tuberculum IHC
Day 5 preoptic area neurosecretory neuron IHC
Adult caudal tuberculum IHC
Adult gigantocellular part of magnocellular preoptic nucleus IHC
Adult magnocellular preoptic nucleus IHC
Adult parvocellular preoptic nucleus IHC
Adult preoptic area neurosecretory neuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-th IHC wz01Tg standard conditions Day 5 dopaminergic neuron
IHC Adult dopaminergic neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife