FIGURE

Fig. 6 S1

ID

ZDB-FIG-170309-20

Publication

Wircer et al., 2017 - Homeodomain protein Otp affects developmental neuropeptide switching in oxytocin neurons associated with a long-term effect on social behavior

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Fig. 6 S1

Single-cell labelling and projection tracing of OXT neurons.

(A) A scheme describing the single-cell labeling method used to trace the projections made by each OXT-ergic neuron. Transgenic Tg(oxt:egfp) embryos were co-microinjected with OXT-specific Gal4 driver, (oxt:Gal4) construct (Blechman et al., 2011) together with constructs harbouring membrane localized caax-tRFP under the control of a multimerized Gal4 upstream activation sequence (10xUAS). Injected embryos were fixed at five dpf, stained for tRFP and EGFP and imaged by a confocal microscope. Injection was calibrated to drives the mosaic expression of caax-tRFP at low efficiency, resulting in single-cell genetic labeling in OXT-ergic neurons. (B) An example of single-cell labeling of an hypothalamo-neurohypophyseal OXT projecting neuron to the posterior pituitary/neurohypophysis. (C) Labeled cells were traced from the 3D confocal Z-Stack using the Fiji image processing and analysis package for 3D tracing and reconstitution with the Simple Neurite Tracer plugin.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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